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Journal of Clinical Microbiology, October 1998, p. 3094-3095, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

PCR-Based Rapid Detection of Mycobacterium tuberculosis in Blood from Immunocompetent Patients with Pulmonary Tuberculosis

Niyaz Ahmed,1,2,* Ashok Kumar Mohanty,1 Utpal Mukhopadhyay,1 Virender Kumar Batish,1 and Sunita Grover1

Molecular Biology Unit, National Dairy Research Institute, Karnal 132001,1 and Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500 007,2 India

Received 12 November 1997/Returned for modification 28 April 1998/Accepted 21 July 1998

A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for the circulating DNA copies of M. tuberculosis complex.

* Corresponding author. Mailing address: Centre for DNA Fingerprinting and Diagnostics, CCMB Campus, Uppal Rd., Hyderabad 500 007, India. Phone: 91-40-7172241. Fax: 91-40-7150008. E-mail: niyaz{at}mailcity.com.

Journal of Clinical Microbiology, October 1998, p. 3094-3095, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • MIRZA, S., RESTREPO, B. I., MCCORMICK, J. B., FISHER-HOCH, S. P. (2003). DIAGNOSIS OF TUBERCULOSIS LYMPHADENITIS USING A POLYMERASE CHAIN REACTION ON PERIPHERAL BLOOD MONONUCLEAR CELLS. Am J Trop Med Hyg 69: 461-465 [Abstract] [Full Text]  


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