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Journal of Clinical Microbiology, October 1998, p. 3103-3104, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Unreliability of Disc Diffusion Test for Screening for Reduced Penicillin Susceptibility in Neisseria meningitidis

Colin Block,1,* Yehudit Davidson,2 and Nathan Keller2

Department of Clinical Microbiology and Infectious Diseases, Hadassah University Hospital, Jerusalem,1 and National Center for Meningococci, Chaim Sheba Medical Center, Tel Hashomer,2 Israel

Received 21 January 1998/Returned for modification 15 May 1998/Accepted 9 July 1998

The 2-U penicillin and 1-µg oxacillin discs proposed for screening meningococci for susceptibility to penicillin were evaluated by using MICs measured by the E test. The discs yielded unacceptably high frequencies of misclassification of susceptibility category and should be abandoned in favor of MIC estimations. An agreed breakpoint for reduced penicillin susceptibility in meningococci is needed for the E test.

* Corresponding author. Mailing address: Department of Clinical Microbiology and Infectious Diseases, Hadassah University Hospital, P.O. Box 12000, Il-91120 Jerusalem, Israel. Phone: 972-2-6776543. Fax: 972-9-7406906. E-mail: block{at}md2.huji.ac.il.

Journal of Clinical Microbiology, October 1998, p. 3103-3104, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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