This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Waris, M. E.
Right arrow Articles by Ruuskanen, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Waris, M. E.
Right arrow Articles by Ruuskanen, O.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 1998, p. 3155-3159, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Diagnosis of Mycoplasma pneumoniae Pneumonia in Children

Matti E. Waris,1,* Pia Toikka,2 Taina Saarinen,2 Simo Nikkari,3 Olli Meurman,4 Raija Vainionpää,1 Jussi Mertsola,2 and Olli Ruuskanen2

Department of Virology,1 Department of Pediatrics,2 and Department of Microbiology,3 University of Turku, and Turku University Hospital Central Laboratory,4 Turku, Finland

Received 5 December 1997/Returned for modification 27 April 1998/Accepted 3 August 1998

We evaluated a commercial immunoglobulin M (IgM)-capture immunoassay for the detection of Mycoplasma pneumoniae infections in 278 pediatric patients with community-acquired, radiographically defined pneumonia. Acute- and convalescent-phase serum samples were collected from all patients and were tested for M. pneumoniae-specific IgM and IgG antibodies by Platelia enzyme immunoassays (Sanofi Diagnostica Pasteur, Marnes la Coquette, France). Nasopharyngeal aspirates (NPAs) were collected at the time of admission to the hospital. A total of 227 NPAs were subjected to the detection of M. pneumoniae DNA by PCR, and 191 NPAs were cultured by using the Pneumofast kit (International Mycoplasma, Signeswere, France). Southern hybridization of PCR products and the IgM test with solid-phase antigen (Serion Immunodiagnostica, Würzburg, Germany) were used for additional confirmation of a positive result, which required agreement of at least two different methods. A total of 24 (9%) confirmed diagnoses of mycoplasma infection were made, 5 (21%) of which were in children <5 years of age. Of the positive children, 24 of 24 (sensitivity, 100%) were positive by the IgM-capture test with convalescent-phase serum, 19 of 24 (79%) were positive by the IgM-capture test with acute-phase serum, 19 of 24 (79%) were positive by IgG serology, 10 of 20 (50%) were positive by PCR, and 8 of 17 (47%) were positive by culture. An additional 5 (of 254) children were positive by the Platelia IgM test alone (specificity, 98%). When the PCR with Southern hybridization result was combined with the IgM-capture test result with the acute-phase sera, the sensitivity of rapid laboratory diagnosis increased to 95%. In conclusion, the IgM serology test was the single most valuable tool for the diagnosis of M. pneumoniae pneumonia in children of any age.

* Corresponding author. Mailing address: Department of Medical Physics and Chemistry, University of Turku, Tykistönkatu 6, FIN-20520 Turku, Finland. Phone: 358 2 333 7059. Fax: 358 2 333 7060. E-mail: matti.waris{at}utu.fi.

Journal of Clinical Microbiology, November 1998, p. 3155-3159, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lahti, E, Peltola, V, Waris, M, Virkki, R, Rantakokko-Jalava, K, Jalava, J, Eerola, E, Ruuskanen, O (2009). Induced sputum in the diagnosis of childhood community-acquired pneumonia. Thorax 64: 252-257 [Abstract] [Full Text]  
  • Martinez, M. A., Ruiz, M., Zunino, E., Luchsinger, V., Avendano, L. F. (2008). Detection of Mycoplasma pneumoniae in adult community-acquired pneumonia by PCR and serology. J Med Microbiol 57: 1491-1495 [Abstract] [Full Text]  
  • Sidal, M., Kilic, A., Unuvar, E., Oguz, F., Onel, M., Agacfidan, A., Aydin, D., Koksalan, K., Beka, H. (2007). Frequency of Clamydia pneumoniae and Mycoplasma pneumoniae Infections in Children. J Trop Pediatr 53: 225-231 [Abstract] [Full Text]  
  • Yamazaki, T., Narita, M., Sasaki, N., Kenri, T., Arakawa, Y., Sasaki, T. (2006). Comparison of PCR for Sputum Samples Obtained by Induced Cough and Serological Tests for Diagnosis of Mycoplasma pneumoniae Infection in Children. CVI 13: 708-710 [Abstract] [Full Text]  
  • Johnston, S. L., Martin, R. J. (2005). Chlamydophila pneumoniae and Mycoplasma pneumoniae: A Role in Asthma Pathogenesis?. Am. J. Respir. Crit. Care Med. 172: 1078-1089 [Abstract] [Full Text]  
  • Beersma, M. F. C., Dirven, K., van Dam, A. P., Templeton, K. E., Claas, E. C. J., Goossens, H. (2005). Evaluation of 12 Commercial Tests and the Complement Fixation Test for Mycoplasma pneumoniae-Specific Immunoglobulin G (IgG) and IgM Antibodies, with PCR Used as the "Gold Standard". J. Clin. Microbiol. 43: 2277-2285 [Abstract] [Full Text]  
  • Raggam, R. B., Leitner, E., Berg, J., Muhlbauer, G., Marth, E., Kessler, H. H. (2005). Single-Run, Parallel Detection of DNA from Three Pneumonia-Producing Bacteria by Real-Time Polymerase Chain Reaction. J. Mol. Diagn. 7: 133-138 [Abstract] [Full Text]  
  • Pelton, S. I., Hammerschlag, M. R. (2005). Overcoming Current Obstacles in the Management of Bacterial Community-Acquired Pneumonia in Ambulatory Children. CLIN PEDIATR 44: 1-17  
  • Daxboeck, F., Blacky, A., Seidl, R., Krause, R., Assadian, O. (2004). Diagnosis, Treatment, and Prognosis of Mycoplasma pneumoniae Childhood Encephalitis: Systematic Review of 58 Cases. J Child Neurol 19: 865-871 [Abstract]  
  • Talkington, D. F., Shott, S., Fallon, M. T., Schwartz, S. B., Thacker, W. L. (2004). Analysis of Eight Commercial Enzyme Immunoassay Tests for Detection of Antibodies to Mycoplasma pneumoniae in Human Serum. CVI 11: 862-867 [Abstract] [Full Text]  
  • Roth, S. B., Jalava, J., Ruuskanen, O., Ruohola, A., Nikkari, S. (2004). Use of an Oligonucleotide Array for Laboratory Diagnosis of Bacteria Responsible for Acute Upper Respiratory Infections. J. Clin. Microbiol. 42: 4268-4274 [Abstract] [Full Text]  
  • Michelow, I. C., Olsen, K., Lozano, J., Duffy, L. B., McCracken, G. H., Hardy, R. D. (2004). Diagnostic Utility and Clinical Significance of Naso- and Oropharyngeal Samples Used in a PCR Assay To Diagnose Mycoplasma pneumoniae Infection in Children with Community-Acquired Pneumonia. J. Clin. Microbiol. 42: 3339-3341 [Abstract] [Full Text]  
  • Loens, K., Ursi, D., Goossens, H., Ieven, M. (2003). Molecular Diagnosis of Mycoplasma pneumoniae Respiratory Tract Infections. J. Clin. Microbiol. 41: 4915-4923 [Full Text]  
  • Templeton, K. E., Scheltinga, S. A., Graffelman, A. W., van Schie, J. M., Crielaard, J. W., Sillekens, P., van den Broek, P. J., Goossens, H., Beersma, M. F. C., Claas, E. C. J. (2003). Comparison and Evaluation of Real-Time PCR, Real-Time Nucleic Acid Sequence-Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae. J. Clin. Microbiol. 41: 4366-4371 [Abstract] [Full Text]  
  • Neumayr, L., Lennette, E., Kelly, D., Earles, A., Embury, S., Groncy, P., Grossi, M., Grover, R., McMahon, L., Swerdlow, P., Waldron, P., Vichinsky, E. (2003). Mycoplasma Disease and Acute Chest Syndrome in Sickle Cell Disease. Pediatrics 112: 87-95 [Abstract] [Full Text]  
  • Virkki, R, Juven, T, Rikalainen, H, Svedstrom, E, Mertsola, J, Ruuskanen, O (2002). Differentiation of bacterial and viral pneumonia in children. Thorax 57: 438-441 [Abstract] [Full Text]  
  • Petitjean, J., Vabret, A., Gouarin, S., Freymuth, F. (2002). Evaluation of Four Commercial Immunoglobulin G (IgG)- and IgM-Specific Enzyme Immunoassays for Diagnosis of Mycoplasma pneumoniae Infections. J. Clin. Microbiol. 40: 165-171 [Abstract] [Full Text]  
  • Dorigo-Zetsma, J. W., Verkooyen, R. P., van Helden, H. P., van der Nat, H., van den Bosch, J. M. (2001). Molecular Detection of Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia Requiring Hospitalization. J. Clin. Microbiol. 39: 1184-1186 [Abstract] [Full Text]  
  • Thacker, W. L., Talkington, D. F. (2000). Analysis of Complement Fixation and Commercial Enzyme Immunoassays for Detection of Antibodies to Mycoplasma pneumoniae in Human Serum. CVI 7: 778-780 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»