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Journal of Clinical Microbiology, November 1998, p. 3182-3187, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Sequencing of Escherichia coli O111 O-Antigen Gene Cluster and Identification of O111-Specific Genes

Lei Wang, Heather Curd, Wenjia Qu, and Peter R. Reeves*

Department of Microbiology, The University of Sydney, New South Wales 2006, Australia

Received 18 May 1998/Returned for modification 7 July 1998/Accepted 24 July 1998

Shiga toxin (Stx)-producing Escherichia coli strains of serogroup O111 are the most frequently isolated non-O157 strains causing outbreaks of gastroenteritis with hemolytic-uremic syndrome. The O111 O-antigen gene cluster had been cloned and about half of it has been sequenced; we have now sequenced the remainder of the gene cluster, which is 12.5 kb in length and which comprises 11 genes. On the basis of sequence similarity, we have identified all the O-antigen genes expected, including five sugar biosynthetic pathway genes, three transferase genes, the O-unit flippase gene, and the O-antigen polymerase gene. By PCR testing with E. coli strains representing all 166 O-antigen forms, some randomly selected gram-negative bacteria, and Salmonella enterica serovar Adelaide, we showed that four O-antigen genes are highly specific to O111. This work provides the basis for a sensitive test for the rapid detection of E. coli O111. This is important both for decisions related to patient care, because early treatment may reduce the risk of life-threatening complications, and for the detection of sources of contamination.

* Corresponding author. Mailing address: Department of Microbiology (G08), The University of Sydney, New South Wales 2006, Australia. Phone: (612) 9351 2536. Fax: (612) 9351 4571. E-mail: reeves{at}angis.su.oz.au.

Journal of Clinical Microbiology, November 1998, p. 3182-3187, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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