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Journal of Clinical Microbiology, November 1998, p. 3193-3197, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Legionella Species by Random Amplified Polymorphic DNA Profiles

François Lo Presti,* Serge Riffard, François Vandenesch, and Jerome Etienne

Centre National de Référence des Legionella, EA1655, Faculté de Médecine R. T. H. Laennec, 69372 Lyon Cedex 08, France

Received 21 January 1998/Returned for modification 26 May 1998/Accepted 3 August 1998

Random amplified polymorphic DNA (RAPD) was used for the identification of Legionella species. Primer SK2 (5'-CGGCGGCGGCGG-3') and standardized RAPD conditions gave the technique a reproducibility of 93 to 100%, depending on the species tested. Species-specific patterns corresponding to the 42 Legionella species were consequently defined by this method; the patterns were dependent on the recognition of a core of common bands for each species. This specificity was demonstrated by testing 65 type strains and 265 environmental and clinical isolates. No serogroup-specific profiles were obtained. A number of unidentified Legionella isolates potentially corresponding to new species were clustered in four groups. RAPD analysis appears to be a rapid and reproducible technique for identification of Legionella isolates to the species level without further restriction or hybridization.

* Corresponding author. Mailing address: Centre National de Référence des Legionella, EA1655, Faculté de Médecine R. T. H. Laennec, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France. Phone: 33 (0) 478-77-86-57. Fax: 33 (0) 478-77-86-58. E-mail: derba{at}cimac-res.univ-lyon1.fr.

Journal of Clinical Microbiology, November 1998, p. 3193-3197, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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