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Journal of Clinical Microbiology, November 1998, p. 3211-3216, Vol. 36, No. 11
Mycoplasma Laboratory, Neisseria Department,
Statens Serum Institut, Copenhagen, Denmark
Received 13 March 1998/Returned for modification 24 April
1998/Accepted 28 July 1998
An assay which combines the direct detection of Ureaplasma
urealyticum with biovar determination was developed and applied to 618 urogenital specimens. U. urealyticum was detected by
inhibitor-controlled PCR. A 429-bp fragment of the urease gene was
amplified. The amplicons were labelled with digoxigenin during PCR.
Biovar determination was performed by liquid hybridization with
biotin-labelled biovar-specific probes, and the hybrids were detected
with peroxidase-conjugated sheep anti-digoxigenin immunoglobulin G Fab
fragments. Results of PCR and culture for 453 urogenital specimens from
women and 105 urethral specimens from men could be compared. Among the
specimens from women, 63% were PCR positive as well as culture
positive, 0.9% were positive only by PCR, and 4% were positive only
by culture. Among the specimens from men, 15% were PCR positive as
well as culture positive, 1% were positive only by PCR, and 9% were
positive only by culture. By using culture as the reference method, the PCR had a sensitivity of 94% and a specificity of 98% when applied to
specimens from women and a sensitivity of 64% and a specificity of
99% when applied to specimens from men. Overall, 80% of the PCR-positive specimens contained biovar 1,13.5% contained biovar 2, and 6.5% contained both biovars.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Detection of Ureaplasma urealyticum by
PCR and Biovar Determination by Liquid Hybridization
*
Corresponding author. Mailing address: Statens Serum
Institut, Neisseria Department, Artillerivej 5, DK-2300 Copenhagen S, Denmark. Phone: 45 32 68 3475. Fax: 45 32 68 3862. E-mail:
knp{at}ssi.dk.
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