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Journal of Clinical Microbiology, November 1998, p. 3297-3302, Vol. 36, No. 11
Departamento de Biología Funcional,
Received 26 May 1998/Returned for modification 7 July 1998/Accepted 28 July 1998
A series of 74 Yersinia enterocolitica clinical strains
collected in a Spanish region and 10 reference strains, assigned to nine serotypes and five biotypes, were analyzed by ribotyping procedures. Riboprobing, performed separately with HindIII
and BglI and using an rrn operon as the probe,
generated 13 and 11 ribotypes (discrimination index [DI] = 0.56 and 0.55), respectively. PCR ribotyping, performed with primers
complementary to conserved regions of 16S and 23S rRNA genes, generated
13 ribotypes (DI = 0.56). A combination of data from the three
procedures allowed for further discrimination into 17 combined
ribotypes (DI = 0.83). The dendrogram obtained by cluster analysis
of data from riboprobing indicated a high heterogeneity of the
ribosomal DNA regions of the strains under study (similarities between
10 and 92%), which were grouped into three clusters at a similarity
level of 0.32. The major cluster included 10 branches, and 7 of these
formed a subcluster (similarity coefficient, >83%) represented by
strains of serotype O:3 and biotype 2, 3, or 4. The second cluster
included four branches, represented by strains belonging to seven
non-O:3 serotypes, biotypes 1A and 2, and two of these branches
included pyrazinamidase-positive as well as pyrazinamidase-negative
strains. The remaining three branches, represented by O:3-biotype 4 strains, formed a third cluster weakly related to the others. Data from this study showed that Y. enterocolitica O:3 organisms
assigned to a prevalent and endemic lineage and non-O:3 organisms
assigned to three other less-frequent lineages are circulating and
causing human disease in the Spanish region under study.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Genetic Heterogeneity of Clinical Strains of Yersinia
enterocolitica Traced by Ribotyping and Relationships between
Ribotypes, Serotypes, and Biotypes
*
Corresponding author. Mailing address: Area de
Microbiología, Facultad de Medicina, C/Julián
Clavería s/n, 33006-Oviedo, Spain. Phone: 34 985103560. Fax: 34-985103148. E-mail: camf{at}sauron.quimica.uniovi.es.
Journal of Clinical Microbiology, November 1998, p. 3297-3302, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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