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Journal of Clinical Microbiology, November 1998, p. 3355-3358, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection and Typing of Borrelia burgdorferi Sensu Lato in Ixodes ricinus Ticks Attached to Human Skin by PCR

Gabriele Liebisch,1,2 Bettina Sohns,1 and Wilfried Bautsch1,*

Institute of Medical Microbiology, Hannover Medical School,1 and Institute of Parasitology, Veterinary School,2 Hannover, Germany

Received 1 May 1998/Returned for modification 2 July 1998/Accepted 4 August 1998

Live Ixodes ricinus ticks attached to humans residing in Germany were examined for borreliae by dark-field microscopy and PCR. Borrelia species were identified by 16S rRNA sequence analysis, which showed the presence of several species, some not yet defined, and a high prevalence of multiply infected ticks.

* Corresponding author. Mailing address: Institute of Medical Microbiology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30623 Hannover, Germany. Phone: (49)-511-532-4342. Fax: (49)-511-532-4366. E-mail: Bautsch.Wilfried{at}MH-Hannover.de.

Journal of Clinical Microbiology, November 1998, p. 3355-3358, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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