This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Louie, M.
Right arrow Articles by Hii, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Louie, M.
Right arrow Articles by Hii, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 1998, p. 3375-3377, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Application of Multiplex PCR for Detection of Non-O157 Verocytotoxin-Producing Escherichia coli in Bloody Stools: Identification of Serogroups O26 and O111

M. Louie,1,2,* S. Read,3 A. E. Simor,1,2 J. Holland,1 L. Louie,1 K. Ziebell,3 J. Brunton,2,4 and J. Hii2

Department of Microbiology, SD Laboratory Services, Sunnybrook Health Science Centre,1 The Toronto Hospital,4 and University of Toronto,2 Toronto, and Health Canada, Health of Animals Laboratory, Guelph,3 Ontario, Canada

Received 27 April 1998/Returned for modification 23 June 1998/Accepted 20 August 1998

Primers were designed to amplify sequences of verocytotoxin genes and eaeA genes of Escherichia coli O26:H11, O111:H8, and O157:H7 in a multiplex PCR assay. This assay successfully detected E. coli O26:H11 in bloody stool specimens in which other enteric pathogens were not detected by culture-based methods. Rapid assays to detect non-O157:H7 verocytotoxin-producing E. coli is important to improve methods for the etiologic diagnosis of hemorrhagic colitis.

* Corresponding author. Mailing address: Department of Microbiology, Sunnybrook Health Science Centre, 2075 Bayview Ave., Room B130, Toronto, Ontario, Canada M4N 3M5. Phone: (416) 480-4243. Fax: (416) 480-6845. E-mail: marie.louie{at}sunnybrook.on.ca.

Journal of Clinical Microbiology, November 1998, p. 3375-3377, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gilmour, M. W., Chui, L., Chiu, T., Tracz, D. M., Hagedorn, K., Tschetter, L., Tabor, H., Ng, L. K., Louie, M. (2009). Isolation and detection of Shiga toxin-producing Escherichia coli in clinical stool samples using conventional and molecular methods. J Med Microbiol 58: 905-911 [Abstract] [Full Text]  
  • Durso, L. M., Bono, J. L., Keen, J. E. (2005). Molecular Serotyping of Escherichia coli O26:H11. Appl. Environ. Microbiol. 71: 4941-4944 [Abstract] [Full Text]  
  • Misawa, N., Kawashima, K., Kondo, F., Ban Mishu Allos, , Blaser, M. J. (2001). DNA diversity of the wla gene cluster among serotype HS:19 and non-HS:19 Campylobacter jejuni strains. Innate Immunity 7: 349-358 [Abstract]  
  • Holland, J. L., Louie, L., Simor, A. E., Louie, M. (2000). PCR Detection of Escherichia coli O157:H7 Directly from Stools: Evaluation of Commercial Extraction Methods for Purifying Fecal DNA. J. Clin. Microbiol. 38: 4108-4113 [Abstract] [Full Text]  
  • Louie, M., Louie, L., Simor, A. E. (2000). The role of DNA amplification technology in the diagnosis of infectious diseases. CMAJ 163: 301-309 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»