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Journal of Clinical Microbiology, November 1998, p. 3385-3388, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of Strategies for Molecular Fingerprinting for Use in the Routine Work of a Mycobacterium Reference Unit

Stuart M. Wilson,* Stephen Goss, and Francis Drobniewski

Public Health Laboratory Service Mycobacterium Reference Unit, Dulwich Hospital, East Dulwich Grove, East Dulwich, London SE22 8QF, United Kingdom

Received 19 May 1998/Returned for modification 22 June 1998/Accepted 18 August 1998

We have investigated the role of two rapid PCR-based typing methods, IS6110-based PCR and spacer-oligonucleotide typing, within a national tuberculosis reference service. The validity of clusters with IS6110 restriction fragment length polymorphism fingerprints with less than 6 bands was also investigated in the context of referred isolates.

* Corresponding author. Mailing address: Public Health Laboratory Service Mycobacterium Reference Unit, Dulwich Hospital, East Dulwich Grove, East Dulwich, London SE22 8QF, United Kingdom. Phone: (44) 181 693 2830. Fax: (44) 171 346 6477. E-mail: stuart.wilson{at}kcl.ac.uk.

Journal of Clinical Microbiology, November 1998, p. 3385-3388, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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