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Journal of Clinical Microbiology, November 1998, p. 3392-3395, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of the QUANTIPLEX HIV-1 RNA 2.0 Assay with the AMPLICOR HIV-1 MONITOR 1.0 Assay for Quantitation of Levels of Human Immunodeficiency Virus Type 1 RNA in Plasma of Patients Receiving Stavudine-Didanosine Combination Therapy

Michel Segondy,1,* Jacques Izopet,2 Isabelle Pellegrin,3 Brigitte Montes,1 Beatrice Dumon,3 Christophe Pasquier,2 Martine Peeters,4 Herve J. A. Fleury,3 Jacqueline Puel,2 and Jacques Reynes1

Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier,1 Department of Virology, Toulouse University Hospital, Toulouse,2 Department of Virology, Bordeaux University Hospital, Bordeaux,3 and Retrovirus Laboratory, ORSTOM, Montpellier,4 France

Received 1 May 1998/Returned for modification 23 June 1998/Accepted 20 August 1998

We compared the QUANTIPLEX HIV-1 RNA 2.0 assay with the AMPLICOR HIV-1 MONITOR 1.0 assay for quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma in the Stadi trail, which evaluated a stavudine plus didanosine combination therapy in 52 patients. HIV-1 RNA baseline values measured with AMPLICOR HIV-1 MONITOR 1.0 were significantly higher than those measured with QUANTIPLEX HIV-1 RNA 2.0, and decreases in HIV-1 RNA levels from baseline were also found to be significantly higher when measured with the AMPLICOR HIV-1 MONITOR 1.0 assay. The frequency of HIV-1 RNA levels below the lower limit of quantitation was significantly higher with QUANTIPLEX HIV-1 RNA 2.0 than with AMPLICOR HIV-1 MONITOR 1.0. Reanalysis of these results by an ultrasensitive procedure of AMPLICOR HIV-1 MONITOR 1.0 or by a modified version of the test that included additional primers adapted for non-B HIV-1 clades yielded greater differences between the QUANTIPLEX HIV-1 RNA 2.0 assay and the AMPLICOR HIV-1 MONITOR 1.0 assay. Our results indicate that a valid comparison of the virological efficacies obtained with different antiretroviral drug regimens requires the use of the same viral load quantitation procedure; further standardization between the different HIV-1 RNA quantitation kits is therefore needed.

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôpital Saint-Eloi, Centre Hospitalier Universitaire, 34295 Montpellier Cedex 5, France. Phone: 33 467 337127. Fax: 33 467 337623. E-mail: msegondy{at}worldnet.fr.

Journal of Clinical Microbiology, November 1998, p. 3392-3395, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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