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Journal of Clinical Microbiology, November 1998, p. 3399-3407, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Determination of 16S rRNA Sequences of Enterococci and Application to Species Identification of Nonmotile Enterococcus gallinarum Isolates

Robin Patel,1,* Kerryl E. Piper,1 Mark S. Rouse,1 James M. Steckelberg,1 Jim R. Uhl,2 Peggy Kohner,2 Marlene K. Hopkins,2 Franklin R. Cockerill III,2 and Bruce C. Kline3

Division of Infectious Diseases and Department of Internal Medicine,1 Division of Clinical Microbiology,2 and Division of Biochemistry and Molecular Biology,3 Mayo Clinic and Foundation, Rochester, Minnesota 55902

Received 6 May 1998/Returned for modification 22 June 1998/Accepted 18 August 1998

The 16S rRNA sequences of enterococcal species E. faecium, E. faecalis, E. gallinarum, E. casseliflavus/flavescens, E. dispar, E. pseudoavium, E. sulfureus, E. malodoratus, E. raffinosus, E. cecorum, E. hirae, E. saccharolyticus, E. seriolicida, E. mundtii, E. avium, E. durans, E. columbae, and E. solitarius are presented herein. These data were utilized to confirm the species identification of two nonmotile E. gallinarum isolates which had been previously phenotypically identified as E. faecium. The implications of this finding are discussed.

* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Internal Medicine, Mayo Clinic and Foundation, Rochester, MN 55902. Phone: (507) 255-7762. Fax: (507) 255-7767. E-mail: patel.robin{at}mayo.edu.

Journal of Clinical Microbiology, November 1998, p. 3399-3407, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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