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Journal of Clinical Microbiology, November 1998, p. 3408-3409, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Enhancement of the AMPLICOR Enterovirus PCR Test with a Coprecipitant

E. William Taggart,1,* Carrie L. Byington,2 David R. Hillyard,1,3 John E. Robison,1 and Karen C. Carroll1,3

Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology,1 Department of Pediatrics,2 and Department of Pathology,3 University of Utah Health Sciences Center, Salt Lake City, Utah

Received 3 April 1998/Returned for modification 12 June 1998/Accepted 20 August 1998

The incorporation of a commercially available coprecipitant into the AMPLICOR enterovirus PCR test specimen preparation enhanced the sensitivity and reproducibility of this assay. Fifty-five previously tested archived cerebrospinal fluids (CSF) specimens were tested in a blind study in duplicate with and without Pellet Paint coprecipitant (Novagen, Inc., Madison, Wis.). Of these specimens, 26 had previously been determined to be positive and 29 had previously been determined to be negative. All previously positive CSF specimens were positive when Pellet Paint was used and only 18 were positive without Pellet Paint. No previously negative specimens were positive on repeat testing with or without Pellet Paint. The background signal was not affected by the addition of Pellet Paint. These data support the utility of a coprecipitant in minimizing false-negative results.

* Corresponding author. Mailing address: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108. Phone: (801) 583-2787, ext. 2018. Fax: (801) 584-5207. E-mail: VIROLOGY{at}arup-lab.com.

Journal of Clinical Microbiology, November 1998, p. 3408-3409, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Xu, M., Chan, Y., Fischer, S. H., Remaley, A. T. (2002). Improvement of AMPLICOR Human Immunodeficiency Virus Type 1 Viral Load Test (Version 1.5) by Addition of a Coprecipitant during the RNA Isolation Step. J. Clin. Microbiol. 40: 2616-2617 [Abstract] [Full Text]  
  • Cubero, J., Graham, J. H., Gottwald, T. R. (2001). Quantitative PCR Method for Diagnosis of Citrus Bacterial Canker. Appl. Environ. Microbiol. 67: 2849-2852 [Abstract] [Full Text]  


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