This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Satoh, Y.
Right arrow Articles by Sairenji, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Satoh, Y.
Right arrow Articles by Sairenji, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 1998, p. 3423-3425, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Pretreatment with Restriction Enzyme or Bovine Serum Albumin for Effective PCR Amplification of Epstein-Barr Virus DNA in DNA Extracted from Paraffin-Embedded Gastric Carcinoma Tissue

Yukio Satoh,1 Noriko Takasaka,1 Yoshiko Hoshikawa,1 Mitsuhiko Osaki,2 Satoshi Ohfuji,2 Hisao Ito,2 Nobuaki Kaibara,3 Takeshi Kurata,4 and Takeshi Sairenji1,4,*

Departments of Biosignaling,1 Pathology,2 and Surgery,3 Faculty of Medicine, Tottori University, Yonago 683, and Department of Pathology, National Institute of Infectious Diseases, Tokyo 162,4 Japan

Received 31 December 1997/Returned for modification 10 April 1998/Accepted 3 August 1998

An association between Epstein-Barr virus (EBV) and gastric carcinoma has been studied through the EBV genome present in the carcinoma cells. Recently, we found that EBV DNA in paraffin-embedded gastric carcinoma tissue was detected effectively by PCR after pretreatment of the extracted DNA with a restriction enzyme, BamHI or EcoRI. Here, we show that the PCR amplification was also enhanced by pretreatment of the DNA with other restriction enzymes or with bovine serum albumin and several other proteins. Treatment with these proteins may remove a PCR inhibitor(s) in the DNA samples extracted from the paraffin blocks.

* Corresponding author. Mailing address: Department of Biosignaling, Faculty of Medicine, Tottori University, 86 Nishimachi, Yonago 683, Japan. Phone: 81 (859) 34-8041. Fax: 81 (859) 34-8042. E-mail: sairen{at}grape.med.tottori-u.ac.jp.

Journal of Clinical Microbiology, November 1998, p. 3423-3425, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kase, S., Adachi, H., Osaki, M., Murakami, M., Sairenji, T., Hashimoto, K., Teramoto, H., Yamamoto, S., Makino, H., Shimizu, E., Watanabe, T., Ohsawa, T., Hagari, Y., Mihara, M., Ito, H. (2004). Epstein-Barr Virus-Infected Malignant T/NK-Cell Lymphoma in a Patient with Hypersensitivity to Mosquito Bites. INT J SURG PATHOL 12: 265-272 [Abstract]  
  • Abu Al-Soud, W., Rådström, P. (2000). Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat. J. Clin. Microbiol. 38: 4463-4470 [Abstract] [Full Text]  
  • Pietilä, J., He, Q., Oksi, J., Viljanen, M. K. (2000). Rapid Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi Sensu Stricto by LightCycler Fluorescence Melting Curve Analysis of a PCR Product of the recA Gene. J. Clin. Microbiol. 38: 2756-2759 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»