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Journal of Clinical Microbiology, November 1998, p. 3429-3432, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Fluconazole Disk Diffusion Susceptibility Testing of Candida Species

William R. Kirkpatrick,1 Thomas M. Turner,1 Annette W. Fothergill,2 Dora I. McCarthy,2 Spencer W. Redding,3 Michael G. Rinaldi,1,2 and Thomas F. Patterson1,4,*

Departments of Medicine,1 Pathology,2 and General Dentistry,3 The University of Texas Health Science Center at San Antonio, and Audie Murphy Division, South Texas Veterans Health Care System,4 San Antonio, Texas 78284

Received 24 April 1998/Returned for modification 2 June 1998/Accepted 4 August 1998

We describe a simple procedure for detecting fluconazole-resistant yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or 50-µg fluconazole disks. With 25-µg disks, zones of inhibition of >= 20 mm at 24 h accurately identified 29 of 29 isolates for which MICs were <= 8 µg/ml, and with 50-µg disks, zones of >= 27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were >8 µg/ml were identified by using either disk. Disk diffusion may be a useful screening method for clinical microbiology laboratories.

* Corresponding author. Mailing address: The University of Texas Health Science Center at San Antonio, Department of Medicine, Division of Infectious Diseases, 7703 Floyd Curl Dr., San Antonio, TX 78284-7881. Phone: (210) 567-4823. Fax: (210) 567-4670. E-mail: PATTERSON{at}UTHSCSA.EDU.

Journal of Clinical Microbiology, November 1998, p. 3429-3432, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.