This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kirkpatrick, W. R.
Right arrow Articles by Patterson, T. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kirkpatrick, W. R.
Right arrow Articles by Patterson, T. F.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 1998, p. 3429-3432, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Fluconazole Disk Diffusion Susceptibility Testing of Candida Species

William R. Kirkpatrick,1 Thomas M. Turner,1 Annette W. Fothergill,2 Dora I. McCarthy,2 Spencer W. Redding,3 Michael G. Rinaldi,1,2 and Thomas F. Patterson1,4,*

Departments of Medicine,1 Pathology,2 and General Dentistry,3 The University of Texas Health Science Center at San Antonio, and Audie Murphy Division, South Texas Veterans Health Care System,4 San Antonio, Texas 78284

Received 24 April 1998/Returned for modification 2 June 1998/Accepted 4 August 1998

We describe a simple procedure for detecting fluconazole-resistant yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or 50-µg fluconazole disks. With 25-µg disks, zones of inhibition of >= 20 mm at 24 h accurately identified 29 of 29 isolates for which MICs were <= 8 µg/ml, and with 50-µg disks, zones of >= 27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were >8 µg/ml were identified by using either disk. Disk diffusion may be a useful screening method for clinical microbiology laboratories.

* Corresponding author. Mailing address: The University of Texas Health Science Center at San Antonio, Department of Medicine, Division of Infectious Diseases, 7703 Floyd Curl Dr., San Antonio, TX 78284-7881. Phone: (210) 567-4823. Fax: (210) 567-4670. E-mail: PATTERSON{at}UTHSCSA.EDU.

Journal of Clinical Microbiology, November 1998, p. 3429-3432, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Brown, S., Traczewski, M. (2007). Quality Control Limits for Posaconazole Disk Susceptibility Tests on Mueller-Hinton Agar with Glucose and Methylene Blue. J. Clin. Microbiol. 45: 222-223 [Abstract] [Full Text]  
  • Pfaller, M. A., Hazen, K. C., Messer, S. A., Boyken, L., Tendolkar, S., Hollis, R. J., Diekema, D. J. (2004). Comparison of Results of Fluconazole Disk Diffusion Testing for Candida Species with Results from a Central Reference Laboratory in the ARTEMIS Global Antifungal Surveillance Program. J. Clin. Microbiol. 42: 3607-3612 [Abstract] [Full Text]  
  • Barry, A., Bille, J., Brown, S., Ellis, D., Meis, J., Pfaller, M., Rennie, R., Rinaldi, M., Rogers, T., Traczewski, M. (2003). Quality Control Limits for Fluconazole Disk Susceptibility Tests on Mueller-Hinton Agar with Glucose and Methylene Blue. J. Clin. Microbiol. 41: 3410-3412 [Abstract] [Full Text]  
  • Rubio, M. C., Gil, J., de Ocariz, I. R., Benito, R., Rezusta, A. (2003). Comparison of Results Obtained by Testing with Three Different Agar Media and by the NCCLS M27-A Method for In Vitro Testing of Fluconazole against Candida spp.. J. Clin. Microbiol. 41: 2665-2668 [Abstract] [Full Text]  
  • Matar, M. J., Ostrosky-Zeichner, L., Paetznick, V. L., Rodriguez, J. R., Chen, E., Rex, J. H. (2003). Correlation between E-Test, Disk Diffusion, and Microdilution Methods for Antifungal Susceptibility Testing of Fluconazole and Voriconazole. Antimicrob. Agents Chemother. 47: 1647-1651 [Abstract] [Full Text]  
  • Nelson, S. M., Cartwright, C. P. (2003). Detection of Fluconazole-Resistant Isolates of Candida glabrata by Using an Agar Screen Assay. J. Clin. Microbiol. 41: 2141-2143 [Abstract] [Full Text]  
  • Pfaller, M. A., Diekema, D. J., Messer, S. A., Boyken, L., Hollis, R. J. (2003). Activities of Fluconazole and Voriconazole against 1,586 Recent Clinical Isolates of Candida Species Determined by Broth Microdilution, Disk Diffusion, and Etest Methods: Report from The ARTEMIS Global Antifungal Susceptibility Program, 2001. J. Clin. Microbiol. 41: 1440-1446 [Abstract] [Full Text]  
  • Hadley, S., Martinez, J. A., McDermott, L., Rapino, B., Snydman, D. R. (2002). Real-time antifungal susceptibility screening aids management of invasive yeast infections in immunocompromised patients. J Antimicrob Chemother 49: 415-419 [Abstract] [Full Text]  
  • Chandrasekar, K., Shyla, J. H., Malathi, R. (2000). Can Antitumor Platinum Compounds Be Effective against Candida albicans?--- A Screening Assay Using Disk Diffusion Method. J. Clin. Microbiol. 38: 3905-3905 [Full Text]  
  • Sandven, P. (1999). Detection of Fluconazole-Resistant Candida Strains by a Disc Diffusion Screening Test. J. Clin. Microbiol. 37: 3856-3859 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»