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Journal of Clinical Microbiology, November 1998, p. 3429-3432, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Fluconazole Disk Diffusion Susceptibility Testing of
Candida Species
William R.
Kirkpatrick,1
Thomas M.
Turner,1
Annette W.
Fothergill,2
Dora I.
McCarthy,2
Spencer W.
Redding,3
Michael G.
Rinaldi,1,2 and
Thomas F.
Patterson1,4,*
Departments of
Medicine,1
Pathology,2 and
General
Dentistry,3 The University of Texas Health
Science Center at San Antonio, and
Audie Murphy Division, South
Texas Veterans Health Care System,4 San
Antonio, Texas 78284
Received 24 April 1998/Returned for modification 2 June
1998/Accepted 4 August 1998
We describe a simple procedure for detecting fluconazole-resistant
yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or
50-µg fluconazole disks. With 25-µg disks, zones of inhibition of
20 mm at 24 h accurately identified 29 of 29 isolates for which
MICs were
8 µg/ml, and with 50-µg disks, zones of
27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were
>8 µg/ml were identified by using either disk. Disk diffusion may be
a useful screening method for clinical microbiology laboratories.
*
Corresponding author. Mailing address: The University
of Texas Health Science Center at San Antonio, Department of Medicine, Division of Infectious Diseases, 7703 Floyd Curl Dr., San Antonio, TX
78284-7881. Phone: (210) 567-4823. Fax: (210) 567-4670. E-mail: PATTERSON{at}UTHSCSA.EDU.
Journal of Clinical Microbiology, November 1998, p. 3429-3432, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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