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Journal of Clinical Microbiology, December 1998, p. 3488-3491, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparative Evaluation of Chlamydiazyme, PACE 2, and AMP-CT Assays for Detection of Chlamydia trachomatis in Endocervical Specimens

John L. Wylie,1,2,* Stephen Moses,2,3 Ryan Babcock,1 Ann Jolly,1,dagger Sandra Giercke,1 and Greg Hammond1,2,3

Cadham Provincial Laboratory,1 Manitoba Health, Department of Medical Microbiology, University of Manitoba,2 and Communicable Disease Control, Public Health Branch, Manitoba Health,3 Winnipeg, Manitoba, Canada

Received 17 June 1998/Returned for modification 13 August 1998/Accepted 7 September 1998

We conducted a comparative evaluation of the Chlamydiazyme (Abbott Laboratories), PACE 2 (Gen-Probe), and AMP-CT (Gen-Probe) assays for the detection of Chlamydia trachomatis in endocervical samples. Specimens from 787 females were included in the study. The sensitivities of the PACE 2 and Chlamydiazyme assays in comparison to the results of the AMP-CT assay were 79.3 and 63.4%, respectively. The specificities of the Chlamydiazyme and PACE 2 assays were 100%. All of the positive specimens detected in this study were positive by the AMP-CT assay. On the basis of the final results of the comparison, the prevalence of C. trachomatis in the population was 10.4%. Retesting of specimens whose results were in the intermediate zone by the PACE 2 assay by a probe competition assay identified some additional true-positive specimens. Amplification assay testing of such specimens did not significantly increase the yield. The majority of specimens which tested positive by the AMP-CT assay only were not in the intermediate zone by the PACE 2 assay. We were unable to identify demographic or clinical factors which could predict those individuals who tested positive by amplified tests but not by nonamplified tests. The Gen-Probe PACE 2 assay proved to be superior to the Chlamydiazyme assay for the screening and diagnosis of C. trachomatis infections in female endocervical specimens.

* Corresponding author. Mailing address: Cadham Provincial Laboratory, 750 William Ave., Winnipeg, Manitoba, Canada R3C 3Y1. Phone: (204) 945-7473. Fax: (204) 786-4770. E-mail: jwylie{at}mb.sympatico.ca.

dagger Present address: Bureau of HIV/AIDS and STD, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada K1A 0K9.

Journal of Clinical Microbiology, December 1998, p. 3488-3491, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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