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Journal of Clinical Microbiology, December 1998, p. 3549-3551, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of the Abbott LCx Assay for Detection of Neisseria gonorrhoeae in Endocervical Swab Specimens from Females

Sue C. Kehl,1,* Kristina Georgakas,2 Geoffrey R. Swain,3,4 Gerald Sedmak,4 Stephen Gradus,4 Ajaib Singh,4 and Seth Foldy3,4

Departments of Pathology1 and Family and Community Medicine,3 Medical College of Wisconsin, United/Dynacare Laboratories,2 and City of Milwaukee Health Department,4 Milwaukee, Wisconsin

Received 20 April 1998/Returned for modification 23 July 1998/Accepted 15 September 1998

The Abbott LCx Neisseria gonorrhoeae assay (Abbott Laboratories, Abbott Park, Ill.) uses a ligase chain reaction (LCR) amplification in the LCx probe system for detection of a specific nucleotide sequence in the Opa-encoding gene of N. gonorrhoeae. We evaluated the LCx assay in a comparison with conventional culture employing modified Thayer-Martin media for the detection of N. gonorrhoeae from female endocervical specimens obtained from patients attending a sexually transmitted disease clinic. Discordantly LCR-positive and culture-negative specimens were further evaluated by testing with another LCR assay which used an N. gonorrhoeae-specific pilin probe. Specimens positive by both LCR assays were considered confirmed LCx-positive specimens. A specimen was considered to contain N. gonorrhoeae when it was either culture positive or culture negative and confirmed LCx positive. A total of 403 female endocervical specimens were evaluated. The prevalence of N. gonorrhoeae in this population was 8.7%. The sensitivity and specificity of the LCx assay were 94.3 and 99.4%, and those of culture were 77.1 and 100%, respectively. The Abbott LCx assay is a rapid, sensitive method for detection of N. gonorrhoeae in female endocervical specimens.

* Corresponding author. Mailing address: Department of Pathology, Medical College of Wisconsin, 865 N. 87th St., P.O. Box 26509, Milwaukee, WI 53226-0509. Phone: (414) 257-6192. Fax: (414) 257-7899. E-mail: kskehl{at}post.its.mcw.edu.

Journal of Clinical Microbiology, December 1998, p. 3549-3551, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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