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Journal of Clinical Microbiology, December 1998, p. 3629-3635, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Epidemiology of Ampicillin-Resistant Non-beta -Lactamase-Producing Haemophilus influenzae

Louise Gazagne,1 Catherine Delmas,1 Edouard Bingen,2 and Henri Dabernat1,*

Laboratoire de Microbiologie, Centre Hospitalier Universitaire Purpan, Toulouse,1 and Laboratoire de Microbiologie, Centre Hospitalier Universitaire R. Debré, Paris,2 France

Received 14 April 1998/Returned for modification 1 July 1998/Accepted 7 September 1998

Resistance to ampicillin without beta -lactamase production is not a frequent occurrence among Haemophilus influenzae strains. This kind of resistance is encountered in unencapsulated strains isolated from bronchial secretions and ear, nose, and throat specimens and is exceptional in H. influenzae type b. We studied 29 of these strains from various areas in France and 2 reference strains. Strains were compared by using ribotyping, arbitarily primed PCR with two primers, and pulsed-field gel electrophoresis. Each technique enabled the identification of 20 to 23 different patterns among the 31 strains. The combination of the different patterns for the strains obtained by the different techniques provided 27 distinct profiles. According to these results, it seems that the clonal propagation of these resistant strains does not occur.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Hôpital PURPAN, Place du Dr. Baylac, 31059 Toulouse Cedex, France. Phone: 33-5 61 77 23 57. Fax: 33-5 61 77.23 33. E-mail: dabernat.h{at}chu-toulouse.fr.

Journal of Clinical Microbiology, December 1998, p. 3629-3635, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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