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Journal of Clinical Microbiology, December 1998, p. 3629-3635, Vol. 36, No. 12
Laboratoire de Microbiologie,
Received 14 April 1998/Returned for modification 1 July
1998/Accepted 7 September 1998
Resistance to ampicillin without
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Molecular Epidemiology of Ampicillin-Resistant
Non-
-Lactamase-Producing Haemophilus
influenzae
-lactamase production is not a
frequent occurrence among Haemophilus influenzae
strains. This kind of resistance is encountered in unencapsulated
strains isolated from bronchial secretions and ear, nose, and
throat specimens and is exceptional in H. influenzae type
b. We studied 29 of these strains from various areas in France and 2 reference strains. Strains were compared by using ribotyping,
arbitarily primed PCR with two primers, and pulsed-field gel
electrophoresis. Each technique enabled the identification of 20 to 23 different patterns among the 31 strains. The combination of the
different patterns for the strains obtained by the different techniques
provided 27 distinct profiles. According to these results, it seems
that the clonal propagation of these resistant strains does not occur.
*
Corresponding author. Mailing address: Laboratoire de
Microbiologie, Hôpital PURPAN, Place du Dr. Baylac, 31059 Toulouse Cedex, France. Phone: 33-5 61 77 23 57. Fax: 33-5 61 77.23 33. E-mail: dabernat.h{at}chu-toulouse.fr.
Journal of Clinical Microbiology, December 1998, p. 3629-3635, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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