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Journal of Clinical Microbiology, December 1998, p. 3686-3688, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effect of Serial Passage in Tissue Culture on Sequence of omp1 from Chlamydia trachomatis Clinical Isolates

Diane R. Stothard,* Barbara Van Der Pol, Nancy J. Smith, and Robert B. Jones

Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202

Received 4 June 1998/Returned for modification 23 July 1998/Accepted 3 September 1998

Previous epidemiological studies of Chlamydia trachomatis frequently have required expansion of isolates in tissue culture. The possibility that C. trachomatis omp1 might undergo mutation during such expansion has not been examined systematically. We found no differences in the omp1 sequences from 10 clinical specimens before and after 20 in vitro passages.

* Corresponding author. Mailing address: Department of Medicine, Infectious Diseases, Indiana University School of Medicine, 435 Emerson Hall, 545 Barnhill Dr., Indianapolis, IN 46202. Phone: (317) 278-1791. Fax: (317) 274-1587. E-mail: dstothar{at}iupui.edu.

Journal of Clinical Microbiology, December 1998, p. 3686-3688, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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