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Journal of Clinical Microbiology, December 1998, p. 3694-3697, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Typing of Human Mycobacterium avium Isolates in Italy by IS1245-Based Restriction Fragment Length Polymorphism Analysis

Nicoletta Lari,1 Michela Cavallini,1 Laura Rindi,1 Elisabetta Iona,2 Lanfranco Fattorini,2 and Carlo Garzelli1,*

Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università di Pisa, I-56127 Pisa,1 and Laboratory of Bacteriology and Medical Micology, Istituto Superiore di Sanità, I-00161 Rome,2 Italy

Received 10 June 1998/Returned for modification 2 August 1998/Accepted 18 August 1998

All but 2 of 63 Mycobacterium avium isolates from distinct geographic areas of Italy exhibited markedly polymorphic, multibanded IS1245 restriction fragment length polymorphism (RFLP) patterns; 2 isolates showed the low-number banding pattern typical of bird isolates. By computer analysis, 41 distinct IS1245 patterns and 10 clusters of essentially identical strains were detected; 40% of the 63 isolates showed genetic relatedness, suggesting the existence of a predominant AIDS-associated IS1245 RFLP pattern.

* Corresponding author. Mailing address: Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Via San Zeno, 35/39, I-56127 Pisa, Italy. Phone: 39 050 559427. Fax: 39 050 559462. E-mail: garzelli{at}biomed.unipi.it.

Journal of Clinical Microbiology, December 1998, p. 3694-3697, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.