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Journal of Clinical Microbiology, December 1998, p. 3707-3709, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Multicenter Evaluation of a Modified Protocol for the RAPIDEC Staph System for Direct Identification of Staphylococcus aureus in Blood Cultures

Arjanne van Griethuysen,1,* Anton Buiting,1 Wil Goessens,2 Peter van Keulen,3 Rob Wintermans,4 and Jan Kluytmans3

Department of Clinical Microbiology, St. Elisabeth Hospital, Tilburg,1 Department of Clinical Microbiology St. Ignatius Hospital and Hospital de Baronie, Breda,3 Department of Clinical Microbiology University Hospital Dijkzigt, Rotterdam,2 and Department of Clinical Microbiology St. Franciscus Hospital, Roosendaal, and Hospital Lievensberg, Bergen op Zoom,4 The Netherlands

Received 26 June 1998/Returned for modification 4 August 1998/Accepted 16 September 1998

A modified protocol for the RAPIDEC Staph system (bioMérieux, Marcy-l'Etoile, France) for direct identification of Staphylococcus aureus in blood cultures was evaluated in a multicenter study. A total of 129 blood cultures (BACTEC 9000 Blood Culture System; Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) containing gram-positive cocci in clusters were analyzed by conventional methods and by RAPIDEC Staph in accordance with the manufacturer's protocol and in accordance with a modified protocol. The sensitivity, specificity, and positive and negative predictive values obtained with the manufacturer's protocol were 90.5, 97.7, 95.0, and 95.5%, respectively, and those obtained with the modified protocol were 100, 96.6, 93.3, and 100%, respectively. The modified protocol for the RAPIDEC Staph is easier to perform than the manufacturer's protocol and is very reliable.

* Corresponding author. Mailing address: Department of Clinical Microbiology, St. Elisabeth Hospital, P.O. Box 747, 5000 AS Tilburg, The Netherlands. Phone: (31) 13-5392650. Fax: (31) 13-5441264. E-mail: jkluytmans{at}ignatius.nl.

Journal of Clinical Microbiology, December 1998, p. 3707-3709, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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