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Journal of Clinical Microbiology, December 1998, p. 3710-3712, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Development of a Scheme for Genotyping Helicobacter pylori Based on Allelic Variation in Urease Subunit Genes

Robert J. Owen,1,* Eleanor R. Slater,1 Jacqueline Xerry,1 Tansy M. Peters,2 E. Louise Teare,2 and Andrew Grant3

Laboratory of Enteric Pathogens, Central Public Health Laboratory,1 and PHLS Statistics Unit,3 London, and Public Health Laboratory, Chelmsford,2 United Kingdom

Received 5 May 1998/Returned for modification 6 July 1998/Accepted 23 September 1998

Helicobacter pylori urease subunit genes in 383 isolates from 10 countries were investigated by PCR-restriction fragment length polymorphism (HaeIII) analysis. Eighty-two different ureAB profiles were documented by reference to known sequences. Variation among 51% of strains was accounted for by 10 predominant patterns, which provided a unique framework for categorizing isolates with geographically diverse origins.

* Corresponding author. Mailing address: Helicobacter Reference Unit, Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: (44) 0181 200 4400 ext. 3740. Fax: (44) 0181 905 9929. E-mail: rowen{at}phls.co.uk

Journal of Clinical Microbiology, December 1998, p. 3710-3712, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.