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Journal of Clinical Microbiology, December 1998, p. 3731-3733, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effectiveness of Resins in Neutralizing Antibiotic Activities in Bactec Plus Aerobic/F Culture Medium

J. Spaargaren,1 C. P. A. van Boven,1 and G. P. Voorn2,*

Department of Medical Microbiology, Leiden University Medical Centre, 2300 RC, Leiden,1 and Department of Medical Microbiology, St. Antonius Hospital, 3420 EM, Nieuwegein,2 The Netherlands

Received 17 February 1998/Returned for modification 20 May 1998/Accepted 10 September 1998

Incorporating resins in blood culture media can effectively reduce the activities of several antibiotics. It was shown that the activities of some generally used antibiotics decreased by 80 to 90% within 2 h in Bactec Plus Aerobic/F resin-containing culture medium. Bactec vials containing resins were still found to be positive for bacteria when antibiotics were present. The addition of beta -lactamase shortened the detection time irrespective of the presence of resins.

* Corresponding author. Mailing address: St. Antonius Hospital, Department of Medical Microbiology, P.O. Box 2500, 3420 EM, Nieuwegein, The Netherlands. Phone: 31 30 6092624. Fax: 31 30 6092429. E-mail: mmiazn{at}knmg.nl.

Journal of Clinical Microbiology, December 1998, p. 3731-3733, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.