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Journal of Clinical Microbiology, February 1998, p. 335-339, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Serotyping Scheme for Campylobacter jejuni and
Campylobacter coli Based on Direct Agglutination of
Heat-Stable Antigens
J. A.
Frost,*
A. N.
Oza,
R. T.
Thwaites, and
B.
Rowe
Laboratory of Enteric Pathogens, Central
Public Health Laboratory, London NW9 5HT, United Kingdom
Received 23 July 1997/Returned for modification 24 September
1997/Accepted 14 November 1997
Campylobacter is now the most frequently reported cause of
gastrointestinal disease in England and Wales, yet few isolates are
characterized beyond the genus level. The majority of isolates are
Campylobacter jejuni (90%), with most of the remainder
being Campylobacter coli. We describe an adaptation of the
Penner serotyping scheme in which passive hemagglutination
has been replaced by detection of heat-stable antigens by direct
bacterial agglutination; absorbed antisera are used where appropriate.
This scheme has been used to type 2,407 C. jejuni samples
and 182 C. coli samples isolated in Wales between April
1996 and March 1997. Forty-seven C. jejuni serotypes were
identified, with the 10 most prevalent serotypes accounting for 53% of
the isolates tested; 19% of the isolates were untypeable. Only fifteen
C. coli serotypes were identified, with three serotypes
accounting for 69% of the isolates. This scheme provides a baseline
for epidemiological studies of C. jejuni and C. coli.
*
Corresponding author. Mailing address: Laboratory of
Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 0181 200 4400. Fax: 0181 905 9929. E-mail: jafrost{at}phls.co.uk.
Journal of Clinical Microbiology, February 1998, p. 335-339, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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