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Journal of Clinical Microbiology, February 1998, p. 362-366, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Rapid, Low-Technology MIC Determination with Clinical Mycobacterium tuberculosis Isolates by Using the Microplate Alamar Blue Assay

Scott G. Franzblau,1,* Richard S. Witzig,2 James C. McLaughlin,3 Patricia Torres,4 Guillermo Madico,4 Antonio Hernandez,3 Michelle T. Degnan,3 Mary B. Cook,3 Virginia K. Quenzer,3 Robert M. Ferguson,5 and Robert H. Gilman2,4

GWL Hansen's Disease Center, Baton Rouge, Louisiana1; Johns Hopkins University School of Public Health, Baltimore, Maryland2; Universidad Peruana Cayetano Heredia, Lima, Peru4; and University of New Mexico Health Science Center3 and Scientific Laboratory Division, New Mexico State Department of Health,5 Albuquerque, New Mexico

Received 3 June 1997/Returned for modification 14 July 1997/Accepted 26 September 1997

A colorimetric, microplate-based Alamar Blue assay (MABA) method was used to determine the MICs of isoniazid (INH), rifampin, streptomycin (SM), and ethambutol (EMB) for 34 Peruvian Mycobacterium tuberculosis isolates (including both pansensitive and multidrug-resistant strains) and the H37Rv strain by using bacterial suspensions prepared directly from solid media. Results for all isolates were available within 8 days. Discordant results were observed on initial tests for 3 of 16 INH-susceptible isolates, 5 of 31 EMB-susceptible isolates, and 2 of 4 SM-resistant isolates (by the BACTEC 460 system). The overall agreements between the MICs obtained by MABA and the results obtained with the BACTEC 460 system were 87.9% for initial results and 93.6% after retesting 12 of 17 samples with discrepant results. Interpretation of MABA endpoints improved with technical experience. The MABA is a simple, rapid, low-cost, appropriate technology which does not require expensive instrumentation and which makes use of a nontoxic, temperature-stable reagent.

* Corresponding author. Mailing address: Laboratory Research Branch, GWL Hansen's Disease Center, P.O. Box 25072, Baton Rouge, LA 70894. Phone: (504) 346-5773. Fax: (504) 346-5786. E-mail: franzblau{at}vt8200.vetmed.lsu.edu.

Journal of Clinical Microbiology, February 1998, p. 362-366, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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