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Journal of Clinical Microbiology, February 1998, p. 391-394, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Chlamydia trachomatis by the Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) in Urine Specimens from Men and Women and Endocervical Specimens from Women

Kimberly A. Crotchfelt,1 Barbara Pare,1 Charlotte Gaydos,1 and Thomas C. Quinn1,2,*

Division of Infectious Diseases, Johns Hopkins University, Baltimore, Maryland,1 and National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland2

Received 30 July 1997/Returned for modification 16 September 1997/Accepted 30 October 1997

Molecular biology-based amplification methods are significantly more sensitive than other methods for the detection of Chlamydia trachomatis. The performance characteristics of the new Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) with endocervical and urine specimens were compared to those of culture for patients attending two Baltimore City sexually transmitted disease clinics and a clinic for adolescents. AMP CT uses transcription-mediated amplification (TMA) and hybridization protection assay procedures to qualitatively detect C. trachomatis by targeting a 23S rRNA. Discrepant results between culture-negative and AMP CT-positive specimens were resolved by direct fluorescent-antibody staining of sedimented culture transport medium for elementary bodies and by TMA with 16S rRNA as a target. Following discrepant analysis, for 480 female urine specimens AMP CT had a sensitivity of 93.8% and a specificity of 100%. For 464 male urine specimens, the resolved sensitivity and specificity of AMP CT were 95.6 and 98.7%, respectively. For the 479 endocervical swab specimens the sensitivity of AMP CT was 100% and the specificity was 99.5%. Resolved culture sensitivities of AMP CT for female and male swab specimens were 52.3 and 58.9%, respectively. These results demonstrate that AMP CT is highly sensitive for the detection of C. trachomatis in endocervical specimens and in urine specimens from men and women.

* Corresponding author. Mailing address: Johns Hopkins University, Division of Infectious Diseases, 720 Rutland Ave., Ross Research Building 1159, Baltimore, MD 21205. Phone: (410) 955-3151. Fax: (410) 955-7889. E-mail: tquinn{at}welchlink.welch.jhu.edu.

Journal of Clinical Microbiology, February 1998, p. 391-394, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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