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Journal of Clinical Microbiology, February 1998, p. 391-394, Vol. 36, No. 2
Division of Infectious Diseases, Johns
Hopkins University, Baltimore, Maryland,1
and
National Institute of Allergy and Infectious Diseases,
National Institutes of Health, Bethesda, Maryland2
Received 30 July 1997/Returned for modification 16 September
1997/Accepted 30 October 1997
Molecular biology-based amplification methods are significantly
more sensitive than other methods for the detection of Chlamydia trachomatis. The performance characteristics of the new Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) with endocervical and
urine specimens were compared to those of culture for patients attending two Baltimore City sexually transmitted disease clinics and a
clinic for adolescents. AMP CT uses transcription-mediated amplification (TMA) and hybridization protection assay procedures to
qualitatively detect C. trachomatis by targeting a 23S
rRNA. Discrepant results between culture-negative and AMP CT-positive specimens were resolved by direct fluorescent-antibody staining of
sedimented culture transport medium for elementary bodies and by TMA
with 16S rRNA as a target. Following discrepant analysis, for 480 female urine specimens AMP CT had a sensitivity of 93.8% and a
specificity of 100%. For 464 male urine specimens, the resolved sensitivity and specificity of AMP CT were 95.6 and 98.7%,
respectively. For the 479 endocervical swab specimens the sensitivity
of AMP CT was 100% and the specificity was 99.5%. Resolved culture
sensitivities of AMP CT for female and male swab specimens were 52.3 and 58.9%, respectively. These results demonstrate that AMP CT is
highly sensitive for the detection of C. trachomatis in
endocervical specimens and in urine specimens from men and women.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Detection of Chlamydia trachomatis by the Gen-Probe
AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) in Urine Specimens from
Men and Women and Endocervical Specimens from Women
*
Corresponding author. Mailing address: Johns Hopkins
University, Division of Infectious Diseases, 720 Rutland Ave., Ross
Research Building 1159, Baltimore, MD 21205. Phone: (410) 955-3151. Fax: (410) 955-7889. E-mail: tquinn{at}welchlink.welch.jhu.edu.
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