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Journal of Clinical Microbiology, February 1998, p. 543-547, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of the RapID CB Plus System for Identification of Corynebacterium Species and Other Gram-Positive Rods

Marie K. Hudspeth,1 Sharon Hunt Gerardo,1 Diane M. Citron,1 and Ellie J. C. Goldstein1,2,*

R. M. Alden Research Laboratory, Santa Monica-UCLA Medical Center, Santa Monica, California 90404,1 and UCLA School of Medicine, Los Angeles, California 900242

Received 7 July 1997/Returned for modification 20 July 1997/Accepted 10 November 1997

Due to the difficulty of identifying Corynebacterium spp. with standard methods, we compared them with the RapID CB Plus system (Remel, Lenexa, Kans. [formerly Innovative Diagnostic Systems, Norcross, Ga.]), which consists of 4 carbohydrate and 14 preformed enzyme tests, for the identification of 98 clinical isolates of Corynebacterium sp., other coryneforms, Listeria monocytogenes, and 17 ATCC strains. Forty (95%) of 42 strains of Corynebacterium spp. were accurately identified to the species level by the RapID CB Plus system, and two additional strains of C. striatum were identified with one additional conventional test for lipid requirement. Twenty-seven (75%) of the 36 coryneform strains tested were identified correctly to the species level. However, three of four strains of Brevibacterium sp. and all seven of the L. monocytogenes strains were identified to the genus level only. Actinomyces strains had variable results, and the one strain of Arcanobacterium haemolyticum tested was not identified. Overall, the RapID CB Plus system compared favorably with the conventional methods, was easy to inoculate and interpret, and is promising as a new method for identification of gram-positive bacilli.

* Corresponding author. Mailing address: R. M. Alden Research Laboratory, 2021 Santa Monica Blvd., Suite 640E, Santa Monica, CA 90404. Phone: (310) 315-1511. Fax: (310) 315-3662.

Journal of Clinical Microbiology, February 1998, p. 543-547, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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