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Journal of Clinical Microbiology, February 1998, p. 548-551, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Molecular Approaches to Diagnosis of Pulmonary Diseases Due to
Mycoplasma pneumoniae
Marianne
Abele-Horn,1,*
Ulrich
Busch,1
Hans
Nitschko,1
Enno
Jacobs,2
Ralph
Bax,3
Friederike
Pfaff,1
Beatrix
Schaffer,1 and
Jürgen
Heesemann1
Max von Pettenkofer-Institute,
Ludwig-Maximilians-University, 80336 Munich,1
Institute for Microbiology, Technical University, 01307 Dresden,2 and
Childrens Hospital
Munich-Schwabing, Technical University, 80404 Munich,3 Germany
Received 12 May 1997/Returned for modification 5 August
1997/Accepted 4 November 1997
In this prospective study, the use of a culture-enhanced PCR assay
for the detection of Mycoplasma pneumoniae,
followed by hybridization with a specific probe (MP-HPCR) or without
hybridization (MP-PCR), and the use of a nested PCR (MP-NPCR) were
evaluated. Clinical samples (190 specimens) from 190 patients with
respiratory complaints were incubated in culture broth overnight and
then subjected to PCR. The results of the PCR were compared to those obtained by culture, the direct antigen test, and serologic testing by
microparticle agglutination and by immunoblotting in unclear cases. The
sensitivities were 19 CFU for MP-PCR, 1.9 CFU for MP-HPCR, and
0.019 CFU for MP-NPCR. PCR amplification of the
-globin gene was
possible in 98% of cases: after dilution of the
-globin-negative samples, all samples were reactive. Correlation between negative MP-NPCR results and negative serology results was found in 89% of
cases; a positive correlation was found with 10% of the patients. Samples from three immunocompromised patients were MP-NPCR positive but serologically negative. High respiratory colonization by
M. pneumoniae (>105 CFU/ml) in patients with
acute respiratory disease could be detected by culture, MP-PCR, and
MP-NPCR. These results indicate that MP-PCR and MP-NPCR are
reliable methods for the detection of M. pneumoniae in
respiratory tract samples of patients with respiratory complaints.
*
Corresponding author. Mailing address: Max von
Pettenkofer-Institute, Ludwig-Maximilians-University,
Pettenkoferstrasse 9a, 80336 Munich, Germany. Phone:
49-89-5160-5245. Fax: 49-89-64 270 288. E-mail:
08964270286-0001{at}t-online.de.
Journal of Clinical Microbiology, February 1998, p. 548-551, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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