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Journal of Clinical Microbiology, March 1998, p. 618-623, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Species-Specific and Ubiquitous-DNA-Based Assays
for Rapid Identification of Staphylococcus aureus
Francis
Martineau,1,2
François J.
Picard,1
Paul H.
Roy,1,3
Marc
Ouellette,1,2 and
Michel G.
Bergeron1,2,*
Centre de Recherche en Infectiologie, Centre
Hospitalier Universitaire de Québec (Pavillon CHUL),
Ste-Foy, Québec, Canada G1V 4G2,1 and
Département de Microbiologie2 and
Département de
Biochimie,3 Université Laval, Ste-Foy,
Québec, Canada G1K 7P4
Received 23 June 1997/Returned for modification 15 September
1997/Accepted 1 December 1997
Staphylococcus aureus is the cause of serious
infections in humans, including endocarditis, deep-seated abscesses,
and bacteremia, which lead to toxic and septic shock syndromes. Rapid
and direct identification of this bacterium specifically and
ubiquitously directly from clinical specimens would be useful in
improving the diagnosis of S. aureus infections in the
clinical microbiology laboratory. A wide variety of kits based on
biochemical characteristics efficiently identify S. aureus,
but the rapidity and the accuracy of each of these methods combined
with testing of clinically relevant antibiotic resistance genes need to
be improved. On the basis of hybridization assays with randomly
selected clones from an S. aureus genomic library, we have
identified a chromosomal DNA fragment which is specific for S. aureus and which detected all 82 S. aureus isolates
tested. This 442-bp fragment was sequenced and was used to design a set
of PCR amplification primers. The PCR assay was also specific and
ubiquitous for the identification from bacterial cultures of 195 clinical strains of S. aureus isolated from a variety of
anatomical sites and obtained from hospitals throughout the world. The
PCR assay that we have developed is simple and can be performed in
about 1 h. This DNA-based test provides a novel diagnostic tool
for the diagnosis of S. aureus infections.
*
Corresponding author. Mailing address: Centre de
Recherche en Infectiologie, CHUQ (Pavillon CHUL), 2705 Boul. Laurier,
Ste-Foy, Québec, Canada G1V 4G2. Phone: (418) 654-2705. Fax:
(418) 654-2715. E-mail:
Michel.G.Bergeron{at}crchul.ulaval.ca.
Journal of Clinical Microbiology, March 1998, p. 618-623, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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