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Journal of Clinical Microbiology, March 1998, p. 618-623, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification of Staphylococcus aureus

Francis Martineau,1,2 François J. Picard,1 Paul H. Roy,1,3 Marc Ouellette,1,2 and Michel G. Bergeron1,2,*

Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Québec (Pavillon CHUL), Ste-Foy, Québec, Canada G1V 4G2,1 and Département de Microbiologie2 and Département de Biochimie,3 Université Laval, Ste-Foy, Québec, Canada G1K 7P4

Received 23 June 1997/Returned for modification 15 September 1997/Accepted 1 December 1997

Staphylococcus aureus is the cause of serious infections in humans, including endocarditis, deep-seated abscesses, and bacteremia, which lead to toxic and septic shock syndromes. Rapid and direct identification of this bacterium specifically and ubiquitously directly from clinical specimens would be useful in improving the diagnosis of S. aureus infections in the clinical microbiology laboratory. A wide variety of kits based on biochemical characteristics efficiently identify S. aureus, but the rapidity and the accuracy of each of these methods combined with testing of clinically relevant antibiotic resistance genes need to be improved. On the basis of hybridization assays with randomly selected clones from an S. aureus genomic library, we have identified a chromosomal DNA fragment which is specific for S. aureus and which detected all 82 S. aureus isolates tested. This 442-bp fragment was sequenced and was used to design a set of PCR amplification primers. The PCR assay was also specific and ubiquitous for the identification from bacterial cultures of 195 clinical strains of S. aureus isolated from a variety of anatomical sites and obtained from hospitals throughout the world. The PCR assay that we have developed is simple and can be performed in about 1 h. This DNA-based test provides a novel diagnostic tool for the diagnosis of S. aureus infections.

* Corresponding author. Mailing address: Centre de Recherche en Infectiologie, CHUQ (Pavillon CHUL), 2705 Boul. Laurier, Ste-Foy, Québec, Canada G1V 4G2. Phone: (418) 654-2705. Fax: (418) 654-2715. E-mail: Michel.G.Bergeron{at}crchul.ulaval.ca.

Journal of Clinical Microbiology, March 1998, p. 618-623, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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