This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martineau, F.
Right arrow Articles by Bergeron, M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martineau, F.
Right arrow Articles by Bergeron, M. G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1998, p. 618-623, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification of Staphylococcus aureus

Francis Martineau,1,2 François J. Picard,1 Paul H. Roy,1,3 Marc Ouellette,1,2 and Michel G. Bergeron1,2,*

Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Québec (Pavillon CHUL), Ste-Foy, Québec, Canada G1V 4G2,1 and Département de Microbiologie2 and Département de Biochimie,3 Université Laval, Ste-Foy, Québec, Canada G1K 7P4

Received 23 June 1997/Returned for modification 15 September 1997/Accepted 1 December 1997

Staphylococcus aureus is the cause of serious infections in humans, including endocarditis, deep-seated abscesses, and bacteremia, which lead to toxic and septic shock syndromes. Rapid and direct identification of this bacterium specifically and ubiquitously directly from clinical specimens would be useful in improving the diagnosis of S. aureus infections in the clinical microbiology laboratory. A wide variety of kits based on biochemical characteristics efficiently identify S. aureus, but the rapidity and the accuracy of each of these methods combined with testing of clinically relevant antibiotic resistance genes need to be improved. On the basis of hybridization assays with randomly selected clones from an S. aureus genomic library, we have identified a chromosomal DNA fragment which is specific for S. aureus and which detected all 82 S. aureus isolates tested. This 442-bp fragment was sequenced and was used to design a set of PCR amplification primers. The PCR assay was also specific and ubiquitous for the identification from bacterial cultures of 195 clinical strains of S. aureus isolated from a variety of anatomical sites and obtained from hospitals throughout the world. The PCR assay that we have developed is simple and can be performed in about 1 h. This DNA-based test provides a novel diagnostic tool for the diagnosis of S. aureus infections.

* Corresponding author. Mailing address: Centre de Recherche en Infectiologie, CHUQ (Pavillon CHUL), 2705 Boul. Laurier, Ste-Foy, Québec, Canada G1V 4G2. Phone: (418) 654-2705. Fax: (418) 654-2715. E-mail: Michel.G.Bergeron{at}crchul.ulaval.ca.

Journal of Clinical Microbiology, March 1998, p. 618-623, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lattar, S. M., Tuchscherr, L. P. N., Caccuri, R. L., Centron, D., Becker, K., Alonso, C. A., Barberis, C., Miranda, G., Buzzola, F. R., von Eiff, C., Sordelli, D. O. (2009). Capsule Expression and Genotypic Differences among Staphylococcus aureus Isolates from Patients with Chronic or Acute Osteomyelitis. Infect. Immun. 77: 1968-1975 [Abstract] [Full Text]  
  • Pu, S., Han, F., Ge, B. (2009). Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus Strains from Louisiana Retail Meats. Appl. Environ. Microbiol. 75: 265-267 [Abstract] [Full Text]  
  • Tuchscherr, L. P. N., Buzzola, F. R., Alvarez, L. P., Lee, J. C., Sordelli, D. O. (2008). Antibodies to Capsular Polysaccharide and Clumping Factor A Prevent Mastitis and the Emergence of Unencapsulated and Small-Colony Variants of Staphylococcus aureus in Mice. Infect. Immun. 76: 5738-5744 [Abstract] [Full Text]  
  • Lee, K.-H., Lee, J.-W., Wang, S.-W., Liu, L.-Y., Lee, M.-F., Chuang, S.-T., Shy, Y.-M., Chang, C.-L., Wu, M.-C., Chi, C.-H. (2008). Development of a novel biochip for rapid multiplex detection of seven mastitis-causing pathogens in bovine milk samples. jvdi 20: 463-471 [Abstract] [Full Text]  
  • Ho, P. L., Wong, M. P., Lai, E. L., Chan, K. H., Chiu, S. S. (2008). DNA Typing of Cytological Samples for Retrospective Identification of an Early Case of Panton-Valentine Leucocidin-Positive, Community-Associated Methicillin-Resistant Staphylococcus aureus Pneumonia. J. Clin. Microbiol. 46: 2457-2458 [Abstract] [Full Text]  
  • Hussain, M., von Eiff, C., Sinha, B., Joost, I., Herrmann, M., Peters, G., Becker, K. (2008). eap Gene as Novel Target for Specific Identification of Staphylococcus aureus. J. Clin. Microbiol. 46: 470-476 [Abstract] [Full Text]  
  • Hata, E., Katsuda, K., Kobayashi, H., Nishimori, K., Uchida, I., Higashide, M., Ishikawa, E., Sasaki, T., Eguchi, M. (2008). Bacteriological Characteristics of Staphylococcus aureus Isolates from Humans and Bulk Milk. J DAIRY SCI 91: 564-569 [Abstract] [Full Text]  
  • Ohara-Nemoto, Y., Haraga, H., Kimura, S., Nemoto, T. K. (2008). Occurrence of staphylococci in the oral cavities of healthy adults and nasal oral trafficking of the bacteria. J Med Microbiol 57: 95-99 [Abstract] [Full Text]  
  • Park, Y. K., Koo, H. C., Kim, S. H., Hwang, S. Y., Jung, W. K., Kim, J. M., Shin, S., Kim, R. T., Park, Y. H. (2007). The Analysis of Milk Components and Pathogenic Bacteria Isolated from Bovine Raw Milk in Korea. J DAIRY SCI 90: 5405-5414 [Abstract] [Full Text]  
  • Tuchscherr, L. P. N., Gomez, M. I., Buzzola, F. R., Calvinho, L. F., Lee, J. C., Sordelli, D. O. (2007). Characterization of a New Variant of IS257 That Has Displaced the Capsule Genes within Bovine Isolates of Staphylococcus aureus. Infect. Immun. 75: 5483-5488 [Abstract] [Full Text]  
  • Ishii, Y., Alba, J., Maehara, C., Murakami, H., Matsumoto, T., Tateda, K., Furuya, N., Iwata, M., Yamaguchi, K. (2006). Identification of biochemically atypical Staphylococcus aureus clinical isolates with three automated identification systems.. J Med Microbiol 55: 387-392 [Abstract] [Full Text]  
  • van Duijkeren, E., Wolfhagen, M. J. H. M., Heck, M. E. O. C., Wannet, W. J. B. (2005). Transmission of a Panton-Valentine Leucocidin-Positive, Methicillin-Resistant Staphylococcus aureus Strain between Humans and a Dog. J. Clin. Microbiol. 43: 6209-6211 [Abstract] [Full Text]  
  • Gillespie, B. E., Oliver, S. P. (2005). Simultaneous Detection of Mastitis Pathogens, Staphylococcus aureus, Streptococcus uberis, and Streptococcus agalactiae by Multiplex Real-Time Polymerase Chain Reaction. J DAIRY SCI 88: 3510-3518 [Abstract] [Full Text]  
  • Wannet, W. J. B., Spalburg, E., Heck, M. E. O. C., Pluister, G. N., Tiemersma, E., Willems, R. J. L., Huijsdens, X. W., de Neeling, A. J., Etienne, J. (2005). Emergence of Virulent Methicillin-Resistant Staphylococcus aureus Strains Carrying Panton-Valentine Leucocidin Genes in The Netherlands. J. Clin. Microbiol. 43: 3341-3345 [Abstract] [Full Text]  
  • Herwegh, S., Carnoy, C., Wallet, F., Loiez, C., Courcol, R. J. (2005). Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR. J. Clin. Microbiol. 43: 2462-2464 [Abstract] [Full Text]  
  • Picard, F. J., Ke, D., Boudreau, D. K., Boissinot, M., Huletsky, A., Richard, D., Ouellette, M., Roy, P. H., Bergeron, M. G. (2004). Use of tuf Sequences for Genus-Specific PCR Detection and Phylogenetic Analysis of 28 Streptococcal Species. J. Clin. Microbiol. 42: 3686-3695 [Abstract] [Full Text]  
  • Wannet, W. J. B., Spalburg, E., Heck, M. E. O. C., Pluister, G. N., Willems, R. J. L., de Neeling, A. J. (2004). Widespread Dissemination in The Netherlands of the Epidemic Berlin Methicillin-Resistant Staphylococcus aureus Clone with Low-Level Resistance to Oxacillin. J. Clin. Microbiol. 42: 3077-3082 [Abstract] [Full Text]  
  • Heilmann, F., van der Zanden, A., Reubsaet, F., Wannet, W. (2004). Identification of 2,600 Clinical Methicillin-Resistant Staphylococcus aureus Strains in The Netherlands Yielded Sporadic Cases of Strains Negative for the Species-Specific Sa442 Gene Fragment. J. Clin. Microbiol. 42: 2350-2350 [Full Text]  
  • Strommenger, B., Kettlitz, C., Werner, G., Witte, W. (2003). Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in Staphylococcus aureus. J. Clin. Microbiol. 41: 4089-4094 [Abstract] [Full Text]  
  • Klaassen, C. H. W., de Valk, H. A., Horrevorts, A. M. (2003). Clinical Staphylococcus aureus Isolate Negative for the Sa442 Fragment. J. Clin. Microbiol. 41: 4493-4493 [Full Text]  
  • Sutterlin, K., Englert, R., Schmidt-Wieland, T., Schmitt, J., Reischl, U., Lehn, N. (2003). Sporadic Cases of Staphylococcus aureus Organisms Negative for a Species-Specific 442-Base Pair Chromosomal Fragment. J. Clin. Microbiol. 41: 3449-3449 [Full Text]  
  • Grisold, A. J., Leitner, E., Muhlbauer, G., Marth, E., Kessler, H. H. (2002). Detection of Methicillin-Resistant Staphylococcus aureus and Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real-Time PCR. J. Clin. Microbiol. 40: 2392-2397 [Abstract] [Full Text]  
  • Shrestha, N. K., Tuohy, M. J., Hall, G. S., Isada, C. M., Procop, G. W. (2002). Rapid Identification of Staphylococcus aureus and the mecA Gene from BacT/ALERT Blood Culture Bottles by Using the LightCycler System. J. Clin. Microbiol. 40: 2659-2661 [Abstract] [Full Text]  
  • TAN, T. Y. (2002). A comparison of PCR detection of mecA with two standard methods of oxacillin disk susceptibility testing for coagulase-negative staphylococci. J Med Microbiol 51: 83-85 [Abstract] [Full Text]  
  • Tan, T. Y., Corden, S., Barnes, R., Cookson, B. (2001). Rapid Identification of Methicillin-Resistant Staphylococcus aureus from Positive Blood Cultures by Real-Time Fluorescence PCR. J. Clin. Microbiol. 39: 4529-4531 [Abstract] [Full Text]  
  • Yugueros, J., Temprano, A., Sanchez, M., Luengo, J. M., Naharro, G. (2001). Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism of gap Gene. J. Clin. Microbiol. 39: 3693-3695 [Abstract] [Full Text]  
  • Akineden, O., Annemuller, C., Hassan, A. A., Lammler, C., Wolter, W., Zschock, M. (2001). Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis. CVI 8: 959-964 [Abstract] [Full Text]  
  • Mason, W. J., Blevins, J. S., Beenken, K., Wibowo, N., Ojha, N., Smeltzer, M. S. (2001). Multiplex PCR Protocol for the Diagnosis of Staphylococcal Infection. J. Clin. Microbiol. 39: 3332-3338 [Abstract] [Full Text]  
  • Martineau, F., Picard, F. J., Ke, D., Paradis, S., Roy, P. H., Ouellette, M., Bergeron, M. G. (2001). Development of a PCR Assay for Identification of Staphylococci at Genus and Species Levels. J. Clin. Microbiol. 39: 2541-2547 [Abstract] [Full Text]  
  • Yugueros, J., Temprano, A., Berzal, B., Sánchez, M., Hernanz, C., Luengo, J. M., Naharro, G. (2000). Glyceraldehyde-3-Phosphate Dehydrogenase-Encoding Gene as a Useful Taxonomic Tool for Staphylococcus spp.. J. Clin. Microbiol. 38: 4351-4355 [Abstract] [Full Text]  
  • Martineau, F., Picard, F. J., Grenier, L., Roy, P. H., Ouellette, M., Bergeron, M. G. (2000). Multiplex PCR assays for the detection of clinically relevant antibiotic resistance genes in staphylococci isolated from patients infected after cardiac surgery. J Antimicrob Chemother 46: 527-534 [Abstract] [Full Text]  
  • Martineau, F., Picard, F. J., Ménard, C., Roy, P. H., Ouellette, M., Bergeron, M. G. (2000). Development of a Rapid PCR Assay Specific for Staphylococcus saprophyticus and Application to Direct Detection from Urine Samples. J. Clin. Microbiol. 38: 3280-3284 [Abstract] [Full Text]  
  • Tollersrud, T., Kenny, K., Reitz, A. J. Jr., Lee, J. C. (2000). Genetic and Serologic Evaluation of Capsule Production by Bovine Mammary Isolates of Staphylococcus aureus and Other Staphylococcus spp. from Europe and the United States. J. Clin. Microbiol. 38: 2998-3003 [Abstract] [Full Text]  
  • Bisognano, C., Vaudaux, P., Rohner, P., Lew, D. P., Hooper, D. C. (2000). Induction of Fibronectin-Binding Proteins and Increased Adhesion of Quinolone-Resistant Staphylococcus aureus by Subinhibitory Levels of Ciprofloxacin. Antimicrob. Agents Chemother. 44: 1428-1437 [Abstract] [Full Text]  
  • Reischl, U., Linde, H.-J., Metz, M., Leppmeier, B., Lehn, N. (2000). Rapid Identification of Methicillin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation Using Real-Time Fluorescence PCR. J. Clin. Microbiol. 38: 2429-2433 [Abstract] [Full Text]  
  • Martineau, F., Picard, F. J., Lansac, N., Ménard, C., Roy, P. H., Ouellette, M., Bergeron, M. G. (2000). Correlation between the Resistance Genotype Determined by Multiplex PCR Assays and the Antibiotic Susceptibility Patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob. Agents Chemother. 44: 231-238 [Abstract] [Full Text]  
  • Sordelli, D. O., Buzzola, F. R., Gomez, M. I., Steele-Moore, L., Berg, D., Gentilini, E., Catalano, M., Reitz, A. J., Tollersrud, T., Denamiel, G., Jeric, P., Lee, J. C. (2000). Capsule Expression by Bovine Isolates of Staphylococcus aureus from Argentina: Genetic and Epidemiologic Analyses. J. Clin. Microbiol. 38: 846-850 [Abstract] [Full Text]  
  • Ke, D., Picard, F. J., Martineau, F., Ménard, C., Roy, P. H., Ouellette, M., Bergeron, M. G. (1999). Development of a PCR Assay for Rapid Detection of Enterococci. J. Clin. Microbiol. 37: 3497-3503 [Abstract] [Full Text]  
  • Marcos, J. Y., Soriano, A. C., Salazar, M. S., Moral, C. H., Ramos, S. S., Smeltzer, M. S., Carrasco, G. N. (1999). Rapid Identification and Typing of Staphylococcus aureus by PCR-Restriction Fragment Length Polymorphism Analysis of the aroA Gene. J. Clin. Microbiol. 37: 570-574 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»