This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adu-Bobie, J.
Right arrow Articles by Dougan, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adu-Bobie, J.
Right arrow Articles by Dougan, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1998, p. 662-668, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Intimins alpha , beta , gamma , and delta , Four Intimin Derivatives Expressed by Attaching and Effacing Microbial Pathogens

Jeannette Adu-Bobie,1 Gad Frankel,1,* Christopher Bain,2 Azizedite Guedes Goncalves,3 Luiz R. Trabulsi,3 Gill Douce,1 Stuart Knutton,2 and Gordon Dougan1

Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ,1 and Institute of Child Health, The University of Birmingham B16 8ET,2 United Kingdom, and Departmento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil3

Received 10 July 1997/Returned for modification 15 October 1997/Accepted 19 November 1997

Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesions. A eukaryotic cell-binding domain is located within a 280-amino-acid (Int280) carboxy terminus of intimin polypeptides. Polyclonal antiserum was raised against Int280 from enteropathogenic Escherichia coli (EPEC) serotypes O127:H6 and O114:H2 (anti-Int280-H6 and anti-Int280-H2, respectively), and Western blot analysis was used to explore the immunological relationship between the intimin polypeptides expressed by different clinical EPEC and enterohemorrhagic E. coli (EHEC) isolates, a rabbit diarrheagenic E. coli strain (RDEC-1), and Citrobacter rodentium. Anti-Int280-H6 serum reacted strongly with some EPEC serotypes, whereas anti-Int280-H2 serum reacted strongly with strains belonging to different EPEC and EHEC serotypes, RDEC-1, and C. rodentium. These observations were confirmed by using purified Int280 in an enzyme-linked immunosorbent assay and by immunogold and immunofluorescence labelling of whole bacterial cells. Some bacterial strains were recognized poorly by either antiserum (e.g., EPEC O86:H34 and EHEC O157:H7). By using PCR primers designed on the basis of the intimin-encoding eae gene sequences of serotype O127:H6, O114:H2, and O86:H34 EPEC and serotype O157:H7 EHEC, we could distinguish between different eae gene derivatives. Accordingly, the different intimin types were designated alpha , beta , delta , and gamma , respectively.

* Corresponding author. Mailing address: Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Rd., London SW7 2AZ, United Kingdom. Phone: 44-71-594-5253. Fax: 44-71-594-5255. E-mail: g.frankel{at}ic.ac.uk.

Journal of Clinical Microbiology, March 1998, p. 662-668, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Geue, L., Klare, S., Schnick, C., Mintel, B., Meyer, K., Conraths, F. J. (2009). Analysis of the Clonal Relationship of Serotype O26:H11 Enterohemorrhagic Escherichia coli Isolates from Cattle. Appl. Environ. Microbiol. 75: 6947-6953 [Abstract] [Full Text]  
  • Torres, A. G., Blanco, M., Valenzuela, P., Slater, T. M., Patel, S. D., Dahbi, G., Lopez, C., Barriga, X. F., Blanco, J. E., Gomes, T. A. T., Vidal, R., Blanco, J. (2009). Genes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates. J. Clin. Microbiol. 47: 2442-2451 [Abstract] [Full Text]  
  • Toth, I., Nougayrede, J.-P., Dobrindt, U., Ledger, T. N., Boury, M., Morabito, S., Fujiwara, T., Sugai, M., Hacker, J., Oswald, E. (2009). Cytolethal Distending Toxin Type I and Type IV Genes Are Framed with Lambdoid Prophage Genes in Extraintestinal Pathogenic Escherichia coli. Infect. Immun. 77: 492-500 [Abstract] [Full Text]  
  • Kobayashi, H., Kanazaki, M., Hata, E., Kubo, M. (2009). Prevalence and Characteristics of eae- and stx-Positive Strains of Escherichia coli from Wild Birds in the Immediate Environment of Tokyo Bay. Appl. Environ. Microbiol. 75: 292-295 [Abstract] [Full Text]  
  • Ha, Y., Ha, S. K., Lee, Y. H., Kim, D., Chae, S., Kim, C. H., Ahn, K. K., Kim, O., Chae, C. (2008). Prevalence of tir gene subtypes in Escherichia coli isolates from pigs. Vet Rec. 163: 424-425 [Full Text]  
  • Pradel, N., Bertin, Y., Martin, C., Livrelli, V. (2008). Molecular Analysis of Shiga Toxin-Producing Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome Patients and Dairy Samples in France. Appl. Environ. Microbiol. 74: 2118-2128 [Abstract] [Full Text]  
  • Heinikainen, S., Pohjanvirta, T., Eklund, M., Siitonen, A., Pelkonen, S. (2007). Tracing Shigatoxigenic Escherichia coli O103, O145, and O174 Infections from Farm Residents to Cattle. J. Clin. Microbiol. 45: 3817-3820 [Abstract] [Full Text]  
  • Whale, A. D., Hernandes, R. T., Ooka, T., Beutin, L., Schuller, S., Garmendia, J., Crowther, L., Vieira, M. A. M., Ogura, Y., Krause, G., Phillips, A. D., Gomes, T. A. T., Hayashi, T., Frankel, G. (2007). TccP2-mediated subversion of actin dynamics by EPEC 2 - a distinct evolutionary lineage of enteropathogenic Escherichia coli. Microbiology 153: 1743-1755 [Abstract] [Full Text]  
  • Hajra, T. K., Bag, P. K., Das, S. C., Mukherjee, S., Khan, A., Ramamurthy, T. (2007). Development of a Simple Latex Agglutination Assay for Detection of Shiga Toxin-Producing Escherichia coli (STEC) by Using Polyclonal Antibody against STEC. CVI 14: 600-604 [Abstract] [Full Text]  
  • Mundy, R., Schuller, S., Girard, F., Fairbrother, J. M., Phillips, A. D., Frankel, G. (2007). Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism. Microbiology 153: 959-967 [Abstract] [Full Text]  
  • Chong, Y., Fitzhenry, R., Heuschkel, R., Torrente, F., Frankel, G., Phillips, A. D. (2007). Human intestinal tissue tropism in Escherichia coli O157 : H7 - initial colonization of terminal ileum and Peyer's patches and minimal colonic adhesion ex vivo. Microbiology 153: 794-802 [Abstract] [Full Text]  
  • Ito, K., Iida, M., Yamazaki, M., Moriya, K., Moroishi, S., Yatsuyanagi, J., Kurazono, T., Hiruta, N., Ratchtrachenchai, O.-A. (2007). Intimin Types Determined by Heteroduplex Mobility Assay of Intimin Gene (eae)-Positive Escherichia coli Strains. J. Clin. Microbiol. 45: 1038-1041 [Abstract] [Full Text]  
  • Lacher, D. W., Steinsland, H., Blank, T. E., Donnenberg, M. S., Whittam, T. S. (2007). Molecular Evolution of Typical Enteropathogenic Escherichia coli: Clonal Analysis by Multilocus Sequence Typing and Virulence Gene Allelic Profiling. J. Bacteriol. 189: 342-350 [Abstract] [Full Text]  
  • Hernandes, R. T., Vieira, M. A. M., Carneiro, S. M., Salvador, F. A., Gomes, T. A. T. (2006). Characterization of Atypical Enteropathogenic Escherichia coli Strains That Express Typical Localized Adherence in HeLa Cells in the Absence of the Bundle-Forming Pilus. J. Clin. Microbiol. 44: 4214-4217 [Abstract] [Full Text]  
  • Blanco, M., Blanco, J. E., Dahbi, G., Mora, A., Alonso, M. P., Varela, G., Gadea, M. P., Schelotto, F., Gonzalez, E. A., Blanco, J. (2006). Typing of intimin (eae) genes from enteropathogenic Escherichia coli (EPEC) isolated from children with diarrhoea in Montevideo, Uruguay: identification of two novel intimin variants ({micro}B and {xi}R/{beta}2B).. J Med Microbiol 55: 1165-1174 [Abstract] [Full Text]  
  • Vaz, T. M. I., Irino, K., Nishimura, L. S., Cergole-Novella, M. C., Guth, B. E. C. (2006). Genetic Heterogeneity of Shiga Toxin-Producing Escherichia coli Strains Isolated in Sao Paulo, Brazil, from 1976 through 2003, as Revealed by Pulsed-Field Gel Electrophoresis.. J. Clin. Microbiol. 44: 798-804 [Abstract] [Full Text]  
  • Geue, L., Selhorst, T., Schnick, C., Mintel, B., Conraths, F. J. (2006). Analysis of the Clonal Relationship of Shiga Toxin-Producing Escherichia coli Serogroup O165:H25 Isolated from Cattle.. Appl. Environ. Microbiol. 72: 2254-2259 [Abstract] [Full Text]  
  • Sinclair, J. F., Dean-Nystrom, E. A., O'Brien, A. D. (2006). The Established Intimin Receptor Tir and the Putative Eucaryotic Intimin Receptors Nucleolin and {beta}1 Integrin Localize at or near the Site of Enterohemorrhagic Escherichia coli O157:H7 Adherence to Enterocytes In Vivo. Infect. Immun. 74: 1255-1265 [Abstract] [Full Text]  
  • Garrido, P., Blanco, M., Moreno-Paz, M., Briones, C., Dahbi, G., Blanco, J., Blanco, J., Parro, V. (2006). STEC-EPEC Oligonucleotide Microarray: A New Tool for Typing Genetic Variants of the LEE Pathogenicity Island of Human and Animal Shiga Toxin-Producing Escherichia coli (STEC) and Enteropathogenic E. coli (EPEC) Strains. Clin. Chem. 52: 192-201 [Abstract] [Full Text]  
  • Garmendia, J., Ren, Z., Tennant, S., Midolli Viera, M. A., Chong, Y., Whale, A., Azzopardi, K., Dahan, S., Sircili, M. P., Franzolin, M. R., Trabulsi, L. R., Phillips, A., Gomes, T. A. T., Xu, J., Robins-Browne, R., Frankel, G. (2005). Distribution of tccP in Clinical Enterohemorrhagic and Enteropathogenic Escherichia coli Isolates. J. Clin. Microbiol. 43: 5715-5720 [Abstract] [Full Text]  
  • Girard, F., Batisson, I., Frankel, G. M., Harel, J., Fairbrother, J. M. (2005). Interaction of Enteropathogenic and Shiga Toxin-Producing Escherichia coli and Porcine Intestinal Mucosa: Role of Intimin and Tir in Adherence. Infect. Immun. 73: 6005-6016 [Abstract] [Full Text]  
  • Kanack, K. J., Crawford, J. A., Tatsuno, I., Karmali, M. A., Kaper, J. B. (2005). SepZ/EspZ Is Secreted and Translocated into HeLa Cells by the Enteropathogenic Escherichia coli Type III Secretion System. Infect. Immun. 73: 4327-4337 [Abstract] [Full Text]  
  • Adams, T. M., Wentzel, A., Kolmar, H. (2005). Intimin-Mediated Export of Passenger Proteins Requires Maintenance of a Translocation-Competent Conformation. J. Bacteriol. 187: 522-533 [Abstract] [Full Text]  
  • Hyma, K. E., Lacher, D. W., Nelson, A. M., Bumbaugh, A. C., Janda, J. M., Strockbine, N. A., Young, V. B., Whittam, T. S. (2005). Evolutionary Genetics of a New Pathogenic Escherichia Species: Escherichia albertii and Related Shigella boydii Strains. J. Bacteriol. 187: 619-628 [Abstract] [Full Text]  
  • Torres, A. G., Zhou, X., Kaper, J. B. (2005). Adherence of Diarrheagenic Escherichia coli Strains to Epithelial Cells. Infect. Immun. 73: 18-29 [Full Text]  
  • Gartner, J. F., Schmidt, M. A. (2004). Comparative Analysis of Locus of Enterocyte Effacement Pathogenicity Islands of Atypical Enteropathogenic Escherichia coli. Infect. Immun. 72: 6722-6728 [Abstract] [Full Text]  
  • Mora, A., Blanco, M., Blanco, J. E., Alonso, M. P., Dhabi, G., Thomson-Carter, F., Usera, M. A., Bartolome, R., Prats, G., Blanco, J. (2004). Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157:H7 Isolates from Humans and Animals in Spain: Identification and Characterization of Two Predominating Phage Types (PT2 and PT8). J. Clin. Microbiol. 42: 4007-4015 [Abstract] [Full Text]  
  • Kuhne, S. A., Hawes, W. S., La Ragione, R. M., Woodward, M. J., Whitelam, G. C., Gough, K. C. (2004). Isolation of Recombinant Antibodies against EspA and Intimin of Escherichia coli O157:H7. J. Clin. Microbiol. 42: 2966-2976 [Abstract] [Full Text]  
  • Newton, H. J., Sloan, J., Bennett-Wood, V., Adams, L. M., Robins-Browne, R. M., Hartland, E. L. (2004). Contribution of Long Polar Fimbriae to the Virulence of Rabbit-Specific Enteropathogenic Escherichia coli. Infect. Immun. 72: 1230-1239 [Abstract] [Full Text]  
  • Beutin, L., Krause, G., Zimmermann, S., Kaulfuss, S., Gleier, K. (2004). Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated from Human Patients in Germany over a 3-Year Period. J. Clin. Microbiol. 42: 1099-1108 [Abstract] [Full Text]  
  • Blanco, M., Blanco, J. E., Blanco, J., de Carvalho, V. M., Onuma, D. L., de Castro, A. F. P. (2004). Typing of Intimin (eae) Genes in Attaching and Effacing Escherichia coli Strains from Monkeys. J. Clin. Microbiol. 42: 1382-1383 [Full Text]  
  • Pickett, C. L., Lee, R. B., Eyigor, A., Elitzur, B., Fox, E. M., Strockbine, N. A. (2004). Patterns of Variations in Escherichia coli Strains That Produce Cytolethal Distending Toxin. Infect. Immun. 72: 684-690 [Abstract] [Full Text]  
  • Blanco, M., Blanco, J. E., Mora, A., Dahbi, G., Alonso, M. P., Gonzalez, E. A., Bernardez, M. I., Blanco, J. (2004). Serotypes, Virulence Genes, and Intimin Types of Shiga Toxin (Verotoxin)-Producing Escherichia coli Isolates from Cattle in Spain and Identification of a New Intimin Variant Gene (eae-{xi}). J. Clin. Microbiol. 42: 645-651 [Abstract] [Full Text]  
  • Teneberg, S., Angstrom, J., Ljungh, A. (2004). Carbohydrate recognition by enterohemorrhagic Escherichia coli: characterization of a novel glycosphingolipid from cat small intestine. Glycobiology 14: 187-196 [Abstract] [Full Text]  
  • Bertin, Y., Boukhors, K., Livrelli, V., Martin, C. (2004). Localization of the Insertion Site and Pathotype Determination of the Locus of Enterocyte Effacement of Shiga Toxin-Producing Escherichia coli Strains. Appl. Environ. Microbiol. 70: 61-68 [Abstract] [Full Text]  
  • Blanco, J. E., Blanco, M., Alonso, M. P., Mora, A., Dahbi, G., Coira, M. A., Blanco, J. (2004). Serotypes, Virulence Genes, and Intimin Types of Shiga Toxin (Verotoxin)-Producing Escherichia coli Isolates from Human Patients: Prevalence in Lugo, Spain, from 1992 through 1999. J. Clin. Microbiol. 42: 311-319 [Abstract] [Full Text]  
  • Ramachandran, V., Brett, K., Hornitzky, M. A., Dowton, M., Bettelheim, K. A., Walker, M. J., Djordjevic, S. P. (2003). Distribution of Intimin Subtypes among Escherichia coli Isolates from Ruminant and Human Sources. J. Clin. Microbiol. 41: 5022-5032 [Abstract] [Full Text]  
  • Jenkins, C., Willshaw, G. A., Evans, J., Cheasty, T., Chart, H., Shaw, D. J., Dougan, G., Frankel, G., Smith, H. R. (2003). Subtyping of virulence genes in verocytotoxin-producing Escherichia coli (VTEC) other than serogroup O157 associated with disease in the United Kingdom. J Med Microbiol 52: 941-947 [Abstract] [Full Text]  
  • Kobayashi, H., Miura, A., Hayashi, H., Ogawa, T., Endo, T., Hata, E., Eguchi, M., Yamamoto, K. (2003). Prevalence and Characteristics of eae-Positive Escherichia coli from Healthy Cattle in Japan. Appl. Environ. Microbiol. 69: 5690-5692 [Abstract] [Full Text]  
  • Anjum, M. F., Lucchini, S., Thompson, A., Hinton, J. C. D., Woodward, M. J. (2003). Comparative Genomic Indexing Reveals the Phylogenomics of Escherichia coli Pathogens. Infect. Immun. 71: 4674-4683 [Abstract] [Full Text]  
  • Clarke, S. C., Haigh, R. D., Freestone, P. P. E., Williams, P. H. (2003). Virulence of Enteropathogenic Escherichia coli, a Global Pathogen. Clin. Microbiol. Rev. 16: 365-378 [Abstract] [Full Text]  
  • Beutin, L., Marches, O., Bettelheim, K. A., Gleier, K., Zimmermann, S., Schmidt, H., Oswald, E. (2003). HEp-2 Cell Adherence, Actin Aggregation, and Intimin Types of Attaching and Effacing Escherichia coli Strains Isolated from Healthy Infants in Germany and Australia. Infect. Immun. 71: 3995-4002 [Abstract] [Full Text]  
  • Nielsen, E. M., Andersen, M. T. (2003). Detection and Characterization of Verocytotoxin-Producing Escherichia coli by Automated 5' Nuclease PCR Assay. J. Clin. Microbiol. 41: 2884-2893 [Abstract] [Full Text]  
  • Nunes, E. B., Saridakis, H. O., Irino, K., Pelayo, J. S. (2003). Genotypic and phenotypic characterization of attaching and effacing Escherichia coli (AEEC) isolated from children with and without diarrhoea in Londrina, Brazil. J Med Microbiol 52: 499-504 [Abstract] [Full Text]  
  • Goncalves, N. S., Hale, C., Dougan, G., Frankel, G., MacDonald, T. T. (2003). Binding of Intimin from Enteropathogenic Escherichia coli to Lymphocytes and Its Functional Consequences. Infect. Immun. 71: 2960-2965 [Abstract] [Full Text]  
  • Neves, B. C., Mundy, R., Petrovska, L., Dougan, G., Knutton, S., Frankel, G. (2003). CesD2 of Enteropathogenic Escherichia coli Is a Second Chaperone for the Type III Secretion Translocator Protein EspD. Infect. Immun. 71: 2130-2141 [Abstract] [Full Text]  
  • Neves, B. C., Shaw, R. K., Frankel, G., Knutton, S. (2003). Polymorphisms within EspA Filaments of Enteropathogenic and Enterohemorrhagic Escherichia coli. Infect. Immun. 71: 2262-2265 [Abstract] [Full Text]  
  • Blanco, M., Blanco, J. E., Mora, A., Rey, J., Alonso, J. M., Hermoso, M., Hermoso, J., Alonso, M. P., Dahbi, G., Gonzalez, E. A., Bernardez, M. I., Blanco, J. (2003). Serotypes, Virulence Genes, and Intimin Types of Shiga Toxin (Verotoxin)-Producing Escherichia coli Isolates from Healthy Sheep in Spain. J. Clin. Microbiol. 41: 1351-1356 [Abstract] [Full Text]  
  • Jores, J., Zehmke, K., Eichberg, J., Rumer, L., Wieler, L. H. (2003). Description of a Novel Intimin Variant (Type {zeta}) in the Bovine O84:NM Verotoxin-Producing Escherichia coli Strain 537/89 and the Diagnostic Value of Intimin Typing. Exp. Biol. Med. 228: 370-376 [Abstract] [Full Text]  
  • Naylor, S. W., Low, J. C., Besser, T. E., Mahajan, A., Gunn, G. J., Pearce, M. C., McKendrick, I. J., Smith, D. G. E., Gally, D. L. (2003). Lymphoid Follicle-Dense Mucosa at the Terminal Rectum Is the Principal Site of Colonization of Enterohemorrhagic Escherichia coli O157:H7 in the Bovine Host. Infect. Immun. 71: 1505-1512 [Abstract] [Full Text]  
  • Zhang, W. L., Kohler, B., Oswald, E., Beutin, L., Karch, H., Morabito, S., Caprioli, A., Suerbaum, S., Schmidt, H. (2002). Genetic Diversity of Intimin Genes of Attaching and Effacing Escherichia coli Strains. J. Clin. Microbiol. 40: 4486-4492 [Abstract] [Full Text]  
  • Stevens, M. P., van Diemen, P. M., Dziva, F., Jones, P. W., Wallis, T. S. (2002). Options for the control of enterohaemorrhagic Escherichia coli in ruminants. Microbiology 148: 3767-3778 [Full Text]  
  • COOKSON, A. L., HAYES, C. M., PEARSON, G. R., ROE, J. M., WALES, A. D., WOODWARD, M. J. (2002). Isolation from a sheep of an attaching and effacing Escherichia coli O115:H- with a novel combination of virulence factors. J Med Microbiol 51: 1041-1049 [Abstract] [Full Text]  
  • La Ragione, R. M., McLaren, I. M., Foster, G., Cooley, W. A., Woodward, M. J. (2002). Phenotypic and Genotypic Characterization of Avian Escherichia coli O86:K61 Isolates Possessing a Gamma-Like Intimin. Appl. Environ. Microbiol. 68: 4932-4942 [Abstract] [Full Text]  
  • Fitzhenry, R. J., Reece, S., Trabulsi, L. R., Heuschkel, R., Murch, S., Thomson, M., Frankel, G., Phillips, A. D. (2002). Tissue Tropism of Enteropathogenic Escherichia coli Strains Belonging to the O55 Serogroup. Infect. Immun. 70: 4362-4368 [Abstract] [Full Text]  
  • Reece, S., Simmons, C. P., Fitzhenry, R. J., Batchelor, M., Hale, C., Matthews, S., Phillips, A. D., Dougan, G., Frankel, G. (2002). Mutagenesis of conserved tryptophan residues within the receptor-binding domain of intimin: influence on binding activity and virulence. Microbiology 148: 657-665 [Abstract] [Full Text]  
  • Fitzhenry, R J, Pickard, D J, Hartland, E L, Reece, S, Dougan, G, Phillips, A D, Frankel, G (2002). Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7. Gut 50: 180-185 [Abstract] [Full Text]  
  • Son, W.-G., Graham, T. A., Gannon, V. P. J. (2002). Immunological Characterization of Escherichia coli O157:H7 Intimin {gamma}1. CVI 9: 46-53 [Abstract] [Full Text]  
  • Wentzel, A., Christmann, A., Adams, T., Kolmar, H. (2001). Display of Passenger Proteins on the Surface of Escherichia coli K-12 by the Enterohemorrhagic E. coli Intimin EaeA. J. Bacteriol. 183: 7273-7284 [Abstract] [Full Text]  
  • Goncalves, N. S., Ghaem-Maghami, M., Monteleone, G., Frankel, G., Dougan, G., Lewis, D. J. M., Simmons, C. P., MacDonald, T. T. (2001). Critical Role for Tumor Necrosis Factor Alpha in Controlling the Number of Lumenal Pathogenic Bacteria and Immunopathology in Infectious Colitis. Infect. Immun. 69: 6651-6659 [Abstract] [Full Text]  
  • Deng, W., Li, Y., Vallance, B. A., Finlay, B. B. (2001). Locus of Enterocyte Effacement from Citrobacter rodentium: Sequence Analysis and Evidence for Horizontal Transfer among Attaching and Effacing Pathogens. Infect. Immun. 69: 6323-6335 [Abstract] [Full Text]  
  • Okeke, I. N., Borneman, J. A., Shin, S., Mellies, J. L., Quinn, L. E., Kaper, J. B. (2001). Comparative Sequence Analysis of the Plasmid-Encoded Regulator of Enteropathogenic Escherichia coli Strains. Infect. Immun. 69: 5553-5564 [Abstract] [Full Text]  
  • Ghaem-Maghami, M., Simmons, C. P., Daniell, S., Pizza, M., Lewis, D., Frankel, G., Dougan, G. (2001). Intimin-Specific Immune Responses Prevent Bacterial Colonization by the Attaching-Effacing Pathogen Citrobacter rodentium. Infect. Immun. 69: 5597-5605 [Abstract] [Full Text]  
  • DIBB-FULLER, M.P., BEST, A., STAGG, D.A., COOLEY, W.A., WOODWARD, M.J. (2001). An in-vitro model for studying the interaction of Escherichia coli O157:H7 and other enteropathogens with bovine primary cell cultures. J Med Microbiol 50: 759-769 [Abstract] [Full Text]  
  • Cid, D., Ruiz-Santa-Quiteria, J. A., Marin, I., Sanz, R., Orden, J. A., Amils, R., de la Fuente, R. (2001). Association between intimin (eae) and EspB gene subtypes in attaching and effacing Escherichia coli strains isolated from diarrhoeic lambs and goat kids. Microbiology 147: 2341-2353 [Abstract] [Full Text]  
  • Zhu, C., Agin, T. S., Elliott, S. J., Johnson, L. A., Thate, T. E., Kaper, J. B., Boedeker, E. C. (2001). Complete Nucleotide Sequence and Analysis of the Locus of Enterocyte Effacement from Rabbit Diarrheagenic Escherichia coli RDEC-1. Infect. Immun. 69: 2107-2115 [Abstract] [Full Text]  
  • Mansfield, K. G., Lin, K.-C., Newman, J., Schauer, D., MacKey, J., Lackner, A. A., Carville, A. (2001). Identification of Enteropathogenic Escherichia coli in Simian Immunodeficiency Virus-Infected Infant and Adult Rhesus Macaques. J. Clin. Microbiol. 39: 971-976 [Abstract] [Full Text]  
  • Feng, P., Weagant, S. D., Monday, S. R. (2001). Genetic Analysis for Virulence Factors in Escherichia coli O104:H21 That Was Implicated in an Outbreak of Hemorrhagic Colitis. J. Clin. Microbiol. 39: 24-28 [Abstract] [Full Text]  
  • Kobayashi, H., Shimada, J., Nakazawa, M., Morozumi, T., Pohjanvirta, T., Pelkonen, S., Yamamoto, K. (2001). Prevalence and Characteristics of Shiga Toxin-Producing Escherichia coli from Healthy Cattle in Japan. Appl. Environ. Microbiol. 67: 484-489 [Abstract] [Full Text]  
  • Phillips, A D, Navabpour, S, Hicks, S, Dougan, G, Wallis, T, Frankel, G (2000). Enterohaemorrhagic Escherichia coli O157:H7 target Peyer's patches in humans and cause attaching/effacing lesions in both human and bovine intestine. Gut 47: 377-381 [Abstract] [Full Text]  
  • Hartland, E. L., Huter, V., Higgins, L. M., Goncalves, N. S., Dougan, G., Phillips, A. D., MacDonald, T. T., Frankel, G. (2000). Expression of Intimin gamma from Enterohemorrhagic Escherichia coli in Citrobacter rodentium. Infect. Immun. 68: 4637-4646 [Abstract] [Full Text]  
  • Parissi-Crivelli, A., Parissi-Crivelli, J. M., Girón, J. A. (2000). Recognition of Enteropathogenic Escherichia coli Virulence Determinants by Human Colostrum and Serum Antibodies. J. Clin. Microbiol. 38: 2696-2700 [Abstract] [Full Text]  
  • Phillips, A. D., Giròn, J., Hicks, S., Dougan, G., Frankel, G. (2000). Intimin from enteropathogenic Escherichia coli mediates remodelling of the eukaryotic cell surface. Microbiology 146: 1333-1344 [Abstract] [Full Text]  
  • Oswald, E., Schmidt, H., Morabito, S., Karch, H., Marches, O., Caprioli, A. (2000). Typing of Intimin Genes in Human and Animal Enterohemorrhagic and Enteropathogenic Escherichia coli: Characterization of a New Intimin Variant. Infect. Immun. 68: 64-71 [Abstract] [Full Text]  
  • Delahay, R. M., Knutton, S., Shaw, R. K, Hartland, E. L., Pallen, M. J., Frankel, G. (1999). The Coiled-coil Domain of EspA Is Essential for the Assembly of the Type III Secretion Translocon on the Surface of Enteropathogenic Escherichia coli. J. Biol. Chem. 274: 35969-35974 [Abstract] [Full Text]  
  • Gansheroff, L. J., Wachtel, M. R., O'Brien, A. D. (1999). Decreased Adherence of Enterohemorrhagic Escherichia coli to HEp-2 Cells in the Presence of Antibodies That Recognize the C-Terminal Region of Intimin. Infect. Immun. 67: 6409-6417 [Abstract] [Full Text]  
  • Batchelor, M., Knutton, S., Caprioli, A., Huter, V., Zanial, M., Dougan, G., Frankel, G. (1999). Development of a Universal Intimin Antiserum and PCR Primers. J. Clin. Microbiol. 37: 3822-3827 [Abstract] [Full Text]  
  • Reid, S. D., Betting, D. J., Whittam, T. S. (1999). Molecular Detection and Identification of Intimin Alleles in Pathogenic Escherichia coli by Multiplex PCR. J. Clin. Microbiol. 37: 2719-2722 [Abstract] [Full Text]  
  • Higgins, L. M., Frankel, G., Connerton, I., Gonçalves, N. S., Dougan, G., MacDonald, T. T. (1999). Role of Bacterial Intimin in Colonic Hyperplasia and Inflammation. Science 285: 588-591 [Abstract] [Full Text]  
  • Higgins, L. M., Frankel, G., Douce, G., Dougan, G., MacDonald, T. T. (1999). Citrobacter rodentium Infection in Mice Elicits a Mucosal Th1 Cytokine Response and Lesions Similar to Those in Murine Inflammatory Bowel Disease. Infect. Immun. 67: 3031-3039 [Abstract] [Full Text]  
  • Monteiro-Neto, V., Trabulsi, L. R. (1999). Phenylpropionic Acid Metabolism: a Marker for Enteropathogenic Escherichia coli Clonal Group 2 Strains. J. Clin. Microbiol. 37: 2121-2121 [Full Text]  
  • Adu-Bobie, J., Trabulsi, L. R., Carneiro-Sampaio, M. M. S., Dougan, G., Frankel, G. (1998). Identification of Immunodominant Regions within the C-Terminal Cell Binding Domain of Intimin alpha  and Intimin beta  from Enteropathogenic Escherichia coli. Infect. Immun. 66: 5643-5649 [Abstract] [Full Text]  
  • Paton, A. W., Manning, P. A., Woodrow, M. C., Paton, J. C. (1998). Translocated Intimin Receptors (Tir) of Shiga-Toxigenic Escherichia coli Isolates Belonging to Serogroups O26, O111, and O157 React with Sera from Patients with Hemolytic-Uremic Syndrome and Exhibit Marked Sequence Heterogeneity. Infect. Immun. 66: 5580-5586 [Abstract] [Full Text]  
  • Sinclair, J. F., O'Brien, A. D. (2002). Cell Surface-localized Nucleolin Is a Eukaryotic Receptor for the Adhesin Intimin-gamma of Enterohemorrhagic Escherichia coli O157:H7. J. Biol. Chem. 277: 2876-2885 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»