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Journal of Clinical Microbiology, March 1998, p. 679-683, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of PCR for Diagnosis of Bordetella pertussis and Bordetella parapertussis Infections

Lena Lind-Brandberg,1 Christina Welinder-Olsson,1 Teresa Lagergård,2 John Taranger,3 Birger Trollfors,3 and Gunilla Zackrisson1,*

Departments of Clinical Bacteriology,1 Medical Microbiology and Immunology,2 and Pediatrics,3 Göteborg University, Göteborg, Sweden

Received 5 May 1997/Returned for modification 10 October 1997/Accepted 17 December 1997

PCR, using primers PIp1 and PIp2, was evaluated for the detection of DNA from Bordetella pertussis in bacterial strains and in nasopharyngeal samples from patients with a cough lasting at least 7 days. The assay could detect DNA from 6 CFU of B. pertussis/10 µl of sample. Results of the PCR assay were compared with those of cultures, a determination of serum antibodies against pertussis toxin and filamentous hemagglutinin, and a clinical evaluation of 2,442 coughing episodes. The overall sensitivity of PCR was 65% (623 of 956), which was higher than the sensitivity of cultures (58%) (P < 0.001). Factors influencing the sensitivity of PCR were the interval between the onset of symptoms and sampling and the vaccination status of the patient. The specificity of PCR was 98% (1,451 of 1,486). The positive and negative predictive values were 95 and 81%, respectively. Parapertussis PCR, using primers BPPA and BPPZ, was positive in 11 of 18 culture-positive cases and was confirmed by serology in another 4 cases. In conclusion, PCR is a valuable complement to cultures and can probably replace cultures for diagnosis of B. pertussis and Bordetella parapertussis infections.

* Corresponding author. Mailing address: Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, S-413 46 Göteborg, Sweden. Phone: 46 31 60 46 91. Fax: 46 31 60 47 60. E-mail: Gunilla.Zackrisson{at}sahlgrenska.se.

Journal of Clinical Microbiology, March 1998, p. 679-683, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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