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Journal of Clinical Microbiology, March 1998, p. 734-741, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Update on Pneumocystis carinii f. sp. hominis Typing Based on Nucleotide Sequence Variations in Internal Transcribed Spacer Regions of rRNA Genes

Chao-Hung Lee,1,* Jannik Helweg-Larsen,2 Xing Tang,1 Shaoling Jin,1 Baozheng Li,1 Marilyn S. Bartlett,1 Jang-Jih Lu,3 Bettina Lundgren,4 Jens D. Lundgren,2 Mats Olsson,5 Sebastian B. Lucas,6 Patricia Roux,7 Antonietta Cargnel,8 Chiara Atzori,8 Olga Matos,9 and James W. Smith1

Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana 462021; Departments of Infectious Diseases2 and Clinical Microbiology,4 Hvidovre Hospital, University of Copenhagen, Copenhagen, Denmark; Department of Pathology, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan3; Swedish Institute for Infectious Disease Control, Stockholm, Sweden5; UMDS Department of Histopathology, St. Thomas' Hospital, London, United Kingdom6; Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire Saint-Antoine, Paris, France7; II Divisione Malattie Infettive, Ospedale L. Sacco, Milan, Italy8; and Disciplina de Protozoologia, Instituto de Higiene e Medicina Tropical, Lisbon, Portugal9

Received 21 July 1997/Returned for modification 8 November 1997/Accepted 28 November 1997

Pneumocystis carinii f. sp. hominis isolates from 207 clinical specimens from nine countries were typed based on nucleotide sequence variations in the internal transcribed spacer regions I and II (ITS1 and ITS2, respectively) of rRNA genes. The number of ITS1 nucleotides has been revised from the previously reported 157 bp to 161 bp. Likewise, the number of ITS2 nucleotides has been changed from 177 to 192 bp. The number of ITS1 sequence types has increased from 2 to 15, and that of ITS2 has increased from 3 to 14. The 15 ITS1 sequence types are designated types A through O, and the 14 ITS2 types are named types a through n. A total of 59 types of P. carinii f. sp. hominis were found in this study.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, 1120 South Dr., FH 419, Indianapolis, IN 46202. Phone: (317) 274-2596. Fax: (317) 278-0643. E-mail: chlee{at}iupui.edu.

Journal of Clinical Microbiology, March 1998, p. 734-741, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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