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Journal of Clinical Microbiology, March 1998, p. 814-817, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

PCR Methods for Rapid Identification and Characterization of Actinobacillus seminis Strains

S. Appuhamy,1 J. G. Coote,1 J. C. Low,2 and R. Parton1,*

Division of Infection and Immunity, University of Glasgow, Glasgow, G12 8QQ,1 and Scottish Agricultural College Veterinary Services, Bush Estate, Penicuik EH26 0QE,2 Scotland, United Kingdom

Received 15 May 1997/Returned for modification 23 September 1997/Accepted 18 November 1997

Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced the simplest pattern and could be useful for identification of A. seminis and for its differentiation from related species. REP- and ERIC-PCR could be used for strain differentiation in epidemiological studies of A. seminis.

* Corresponding author. Mailing address: Division of Infection and Immunity, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, Scotland, United Kingdom. Phone: 0141 330 5844. Fax: 0141 330 4600. E-mail: rparton{at}bio.gla.ac.uk.

Journal of Clinical Microbiology, March 1998, p. 814-817, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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