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Journal of Clinical Microbiology, March 1998, p. 820-823, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Simplified Technique for Detection of Significant Bacteriuria by Microscopic Examination of Urine

Celso Luíz Cardoso,1,* Carla Barbosa Muraro,2 Vera Lúcia Dias Siqueira,1 and Márcio Guilhermetti1

Laboratory of Microbiology, Department of Clinical Analyses,1 and Department of Medicine,2 State University of Maringá, 87020-900 Maringá, Paraná, Brazil

Received 7 May 1997/Returned for modification 10 July 1997/Accepted 25 November 1997

A comparative study of microscopic examination of 10 µl (simplified loop technique) and 50 µl (traditional drop technique) of uncentrifuged Gram-stained urine specimens for detection of significant bacteriuria was carried out. The results demonstrated that the 10-µl loop technique can be used as an alternative to the 50-µl drop technique for presumptive diagnosis of urinary-tract infection in bacteriological practice, with the advantages of greater rapidity and ease of performance.

* Corresponding author. Mailing address: Laboratory of Microbiology, Department of Clinical Analyses, State University of Maringá, 5790 Colombo Ave., 87020-900 Maringá, Paraná, Brazil. Phone: 55 44 261-4429. Fax: 55 44 263-5116. E-mail: deres{at}cybertelecom.com.br.

Journal of Clinical Microbiology, March 1998, p. 820-823, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.