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Journal of Clinical Microbiology, March 1998, p. 827-829, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Practical Approach for Detection and Identification of OXA-10-Derived Ceftazidime-Hydrolyzing Extended-Spectrum beta -Lactamases

Haluk Vahaboglu,1,* Recep Ozturk,2 Huriye Akbal,2 Suat Saribas,2 Ozlem Tansel,1 and Figen Coskunkan1

Kocaeli Universitesi, Tip Fakültesi, Kocaeli,1 and İstanbul Universitesi, Cerrahpasa Tip Fakültesi, İstanbul,2 Turkey

Received 10 September 1997/Returned for modification 17 November 1997/Accepted 12 December 1997

A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta -lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of blaOXA was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.

* Corresponding author. Mailing address: KOU Tip Fakultesi, Sopali çiftligi 41900 Derince, Kocaeli, Turkey. Phone: 0262 2395209/138. Fax: 0262 2394463. E-mail: vahabo{at}uedh.com.tr.

Journal of Clinical Microbiology, March 1998, p. 827-829, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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