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Journal of Clinical Microbiology, March 1998, p. 848-849, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection and Direct Typing of Herpes Simplex Virus in Perianal Ulcers of Patients with AIDS by PCR

Maria C. do Nascimento,1 Laura M. Sumita,2 Vanda A. U. F. de Souza,2 and Cláudio S. Pannuti2,*

Instituto de Infectologia Emílio Ribas, São Paulo, SP, 01246-900,1 and Laboratory of Virology (LIMHC) of the Instituto de Medicina Tropical de São Paulo, Department of Infectious Diseases, University of São Paulo School of Medicine, São Paulo, SP, 05403-000,2 Brazil

Received 18 July 1997/Returned for modification 3 October 1997/Accepted 22 December 1997

The presence of herpes simplex virus type 1 (HSV-1) and HSV-2 in perianal ulcerations of 41 AIDS patients was assessed by virus culture and a type-specific PCR-based assay. HSV was isolated from the lesion site in 24 of 41 (58.5%) patients, and HSV DNA was detected by PCR in all 24 (100%) of these specimens. Additionally, PCR was used to detect HSV DNA in 12 of 17 (70.5%) HSV culture-negative samples. Thus, HSV genomic sequences could be demonstrated in 36 of 41 (87.8%) perianal ulcers in this series. Full agreement in HSV typing by either immunodot assay or PCR was seen in 24 samples that were positive by both virus culture and PCR. HSV-2 was demonstrated in 35 of 36 (97.2%) HSV-positive samples.

* Corresponding author. Mailing address: Instituto de Medicina Tropical de São Paulo, Av. Dr. Eneas de Carvalho Aguiar, 470, 05403-000, São Paulo, SP, Brazil. Phone: 11-852-2645. Fax: 11-852-2174. E-mail: cpannuti{at}usp.br.

Journal of Clinical Microbiology, March 1998, p. 848-849, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nascimento, M. C., Ferreira, S., Sabino, E., Hamilton, I., Parry, J., Pannuti, C. S., Mayaud, P. (2007). Performance of the HerpeSelect (Focus) and Kalon Enzyme-Linked Immunosorbent Assays for Detection of Antibodies against Herpes Simplex Virus Type 2 by Use of Monoclonal Antibody-Blocking Enzyme Immunoassay and Clinicovirological Reference Standards in Brazil. J. Clin. Microbiol. 45: 2309-2311 [Abstract] [Full Text]  
  • Nascimento, M. C., de Souza, V. A., Sumita, L. M., Freire, W., Munoz, F., Kim, J., Pannuti, C. S., Mayaud, P. (2007). Comparative Study of Kaposi's Sarcoma-Associated Herpesvirus Serological Assays Using Clinically and Serologically Defined Reference Standards and Latent Class Analysis. J. Clin. Microbiol. 45: 715-720 [Abstract] [Full Text]  
  • Coyle, P. V., Jain, S., Wyatt, D., McCaughey, C., O'Neill, H. J. (2000). Description of a Nonlethal Herpes Simplex Virus Type 1 Glycoprotein D Deletion Mutant Affecting a Site Frequently Used for PCR. CVI 7: 322-324 [Abstract] [Full Text]  


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