This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lina, G.
Right arrow Articles by Etienne, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lina, G.
Right arrow Articles by Etienne, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 1998, p. 1042-1045, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Staphylococcal Superantigenic Toxins by a CD69-Specific Cytofluorimetric Assay Measuring T-Cell Activation

Gerard Lina,1,* Grégoire Cozon,2 Josette Ferrandiz,2 Timothy Greenland,3 François Vandenesch,1 and Jerome Etienne1

Centre National de Référence des Staphylocoques, Faculté de Médecine, UPRES EA1655, 69372 Lyon cedex 08,1 Unité d'Immunologie, Hôpital de la Croix-Rousse, 69317 Lyon cedex 04,2 and Laboratoire d'Immunologie et Biologie Pulmonaire, Hôpital Cardiologique Louis Pradel, 69394 Lyon cedex 03,3 France

Received 8 October 1997/Returned for modification 16 December 1997/Accepted 20 January 1998

The presence of staphylococcal superantigenic toxins in the supernatants of liquid cultures was detected by an easy and rapid method assessing the activation of T lymphocytes by cytofluorimetric measurement of CD69 expression. Staphylococcus aureus cells were grown in Eagle's minimum essential medium supplemented with 5% heat-inactivated fetal calf serum. Supernatant fluids from all S. aureus strains producing superantigen-related toxins, including enterotoxins A to E, toxic shock syndrome toxin, and exfoliative toxins A and B, induced CD69 expression in a significantly higher number of T cells than a cutoff of 2%. This CD69 assay might be used for initial detection of superantigens from S. aureus strains isolated in the context of staphylococcal toxemia or related chronic human diseases such as atopic dermatitis or Kawasaki syndrome.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Faculté de Médecine, rue Guillaume Paradin, 69372 Lyon cedex 08, France. Phone: (33) 47 877.86.57. Fax: (33) 47 877.86.58. E-mail: geralina{at}univ-lyon1.fr.

Journal of Clinical Microbiology, April 1998, p. 1042-1045, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Thomas, D., Dauwalder, O., Brun, V., Badiou, C., Ferry, T., Etienne, J., Vandenesch, F., Lina, G. (2009). Staphylococcus aureus Superantigens Elicit Redundant and Extensive Human V{beta} Patterns. Infect. Immun. 77: 2043-2050 [Abstract] [Full Text]  
  • Cozon, G. J. N., Mbitikon-Kobo, F. M., Fatoohi, F., Spire, M., Grange, J.-D., Kodjikian, L. (2008). Abnormal Cellular Reactivity to Microbial Antigens in Patients with Uveitis. IOVS 49: 2526-2530 [Abstract] [Full Text]  
  • Dauwalder, O., Thomas, D., Ferry, T., Debard, A.-L., Badiou, C., Vandenesch, F., Etienne, J., Lina, G., Monneret, G. (2006). Comparative inflammatory properties of staphylococcal superantigenic enterotoxins SEA and SEG: implications for septic shock. J. Leukoc. Biol. 80: 753-758 [Abstract] [Full Text]  
  • Thomas, D. Y., Jarraud, S., Lemercier, B., Cozon, G., Echasserieau, K., Etienne, J., Gougeon, M.-L., Lina, G., Vandenesch, F. (2006). Staphylococcal Enterotoxin-Like Toxins U2 and V, Two New Staphylococcal Superantigens Arising from Recombination within the Enterotoxin Gene Cluster.. Infect. Immun. 74: 4724-4734 [Abstract] [Full Text]  
  • Lennon, G. P., Sillibourne, J. E., Furrie, E., Doherty, M. J., Kay, R. A. (2000). Antigen Triggering Selectively Increases TCRBV Gene Transcription. J. Immunol. 165: 2020-2027 [Abstract] [Full Text]  
  • Jarraud, S., Cozon, G., Vandenesch, F., Bes, M., Etienne, J., Lina, G. (1999). Involvement of Enterotoxins G and I in Staphylococcal Toxic Shock Syndrome and Staphylococcal Scarlet Fever. J. Clin. Microbiol. 37: 2446-2449 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»