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Journal of Clinical Microbiology, April 1998, p. 1105-1108, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Baculovirus Expression of the Fusion Protein Gene of Bovine Respiratory Syncytial Virus and Utility of the Recombinant Protein in a Diagnostic Enzyme Immunoassay

Manoj K. Pastey and Siba K. Samal*

Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742

Received 26 June 1997/Returned for modification 14 October 1997/Accepted 15 December 1997

The fusion (F) protein of bovine respiratory syncytial virus (BRSV) was expressed by using a baculovirus vector. Antigenicity was tested by immunofluorescence analysis with F-specific monoclonal and polyclonal antibodies. Antibodies to recombinant F protein raised in a rabbit neutralized BRSV and human respiratory syncytial virus infectivity when tested in a plaque reduction assay. The recombinant F protein was evaluated as a source of antigen in an enzyme-linked immunosorbent assay (ELISA), and this ELISA was compared with the virus neutralization (VN) test for detecting BRSV antibodies in 10 consecutive serum samples from four calves vaccinated with a live modified BRSV vaccine and from two nonvaccinated control calves. The ELISA with the baculovirus-expressed F protein as an antigen compared favorably with the VN test and is a rapid, sensitive, and specific method for detecting serum antibodies to BRSV.

* Corresponding author. Mailing address: 8075 Greenmead Dr., VA-MD Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742. Phone: (301) 935-6083, ext. 112. Fax: (301) 935-6079. E-mail: ss5{at}umail.umd.edu.

Journal of Clinical Microbiology, April 1998, p. 1105-1108, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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  • Gulati, B. R., Cameron, K. T., Seal, B. S., Goyal, S. M., Halvorson, D. A., Njenga, M. K. (2000). Development of a Highly Sensitive and Specific Enzyme-Linked Immunosorbent Assay Based on Recombinant Matrix Protein for Detection of Avian Pneumovirus Antibodies. J. Clin. Microbiol. 38: 4010-4014 [Abstract] [Full Text]  


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