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Journal of Clinical Microbiology, April 1998, p. 1109-1112, Vol. 36, No. 4
0095-1137/98/$00.00+0

Sensitivity and Specificity of an Improved Rapid Latex Agglutination Test for Identification of Methicillin-Sensitive and -Resistant Staphylococcus aureus Isolates

Sandra C. Smole,1,2 Elyssa Aronson,2 Annette Durbin,3 Stephen M. Brecher,3,4 and Robert D. Arbeit1,2,4,*

Departments of Medicine1 and Microbiology,4 Boston University School of Medicine, and Infectious Diseases Section, Medical Service,2 and Pathology and Laboratory Medicine,3 Boston Veterans Affairs Medical Center, Boston, Massachusetts 02130

Received 29 August 1997/Returned for modification 10 October 1997/Accepted 23 December 1997

The performance of a second-generation rapid agglutination kit, Slidex Staph Plus (SSP; bioMérieux), was compared to those of the Slidex Staph (SS; bioMérieux), Staphaurex (SRX; Murex Diagnostics), and BBL Staphyloslide (BBL; Becton Dickinson) kits by using 508 clinical isolates composed of 150 methicillin-sensitive Staphylococcus aureus (MSSA) organisms, 154 methicillin-resistant S. aureus (MRSA) organisms, and 204 non-S. aureus Staphylococcus spp. Of the 508 isolates tested, 75% were fresh clinical isolates, with the remainder taken from five different freezer collections. All four agglutination tests had comparable sensitivities for MSSA and MRSA. However, the SS kit was significantly less specific (93.1%) than the three other tests (P > 0.05, McNemar test). These results demonstrate that the new rapid latex agglutination kit, SSP, was more specific for the identification of S. aureus than the previous version and performed comparably to the SRX and BBL kits.

* Corresponding author. Mailing address: Research Service (151), VA Medical Center, 150 S. Huntington Ave., Boston, MA 02130. Phone: (617) 278-4416. Fax: (617) 739-6394. E-mail: rarbeit{at}bu.edu.

Journal of Clinical Microbiology, April 1998, p. 1109-1112, Vol. 36, No. 4
0095-1137/98/$00.00+0


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