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Journal of Clinical Microbiology, April 1998, p. 1117-1121, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Intravitam Diagnosis of Human Rabies by PCR Using Saliva and Cerebrospinal Fluid

P. Crepin,1 L. Audry,1 Y. Rotivel,1 A. Gacoin,2 C. Caroff,2 and H. Bourhy1,*

Rabies Unit, Institut Pasteur, Paris,1 and Regional and University Hospital, Rennes,2 France

Received 3 September 1997/Returned for modification 18 December 1997/Accepted 15 January 1998

An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluorescence assay performed with skin biopsy samples allowed detection of rabies in the nine patients.

* Corresponding author. Mailing address: National Reference Center for Rabies, Institut Pasteur, 75724 Paris Cedex 15, France. Phone: 01 45 68 87 85. Fax: 01 40 61 30 20. E-mail: hbourhy{at}pasteur.fr.

Journal of Clinical Microbiology, April 1998, p. 1117-1121, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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