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Journal of Clinical Microbiology, April 1998, p. 1125-1127, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Development of a Monoclonal Antibody to a Ureaplasma urealyticum Serotype 9 Antigen

Anne Naessens,1,* Xiaoxing Cheng,1,dagger Sabine Lauwers,1 and Janet A. Robertson2

Department of Microbiology, Akademisch Ziekenhuis, Vrije Universiteit Brussel, 1090 Brussels, Belgium,1 and Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6J 1Z9, Canada2

Received 4 August 1997/Returned for modification 10 October 1997/Accepted 23 December 1997

We produced a monoclonal antibody (MAb) to Ureaplasma urealyticum Vancouver, the serotype 9 standard strain. By immunoblotting, this MAb showed a single, 85-kDa band with the homologous serotype and a minor, 100-kDa band with serotype 2 but did not react with any other serotype standard strain. Clinical isolates of U. urealyticum were tested with this MAb and with two sets of polyclonal antisera against the 14 serotype standard strains. The use of MAb 9-2H9 correctly identified certain serotype 9 strains but did not react with wild-type strains lacking the serotype 9 determinant.

* Corresponding author. Mailing address: Department of Microbiology, Akademisch Ziekenhuis, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium. Phone: 32-02-4775000. Fax: 32-02-4775015. E-mail: labomicro{at}az.vub.ac.be.

dagger Present address: Dept. of Veterinary and Biomedical Science, University of Nebraska---Lincoln, Lincoln, NE 68583-0905.

Journal of Clinical Microbiology, April 1998, p. 1125-1127, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Vancutsem, E., Echahidi, F., Van Geel, K., Muyldermans, G., Soetens, O., Naessens, A. (2008). Production of Recombinant Antigens of Ureaplasma parvum Serotypes 3 and 6 for Development of a Serological Assay. CVI 15: 447-451 [Abstract] [Full Text]  
  • Echahidi, F., Muyldermans, G., Lauwers, S., Naessens, A. (2001). Development of an Enzyme-Linked Immunosorbent Assay for Serotyping Ureaplasma urealyticum Strains Using Monoclonal Antibodies. CVI 8: 52-57 [Abstract] [Full Text]  
  • Echahidi, F., Muyldermans, G., Lauwers, S., Naessens, A. (2000). Development of Monoclonal Antibodies against Ureaplasma urealyticum Serotypes and Their Use for Serotyping Clinical Isolates. CVI 7: 563-567 [Abstract] [Full Text]  


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