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Journal of Clinical Microbiology, April 1998, p. 1128-1134, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Application of an Optimized and Highly Discriminatory Method Based on Arbitrarily Primed PCR for Epidemiologic Analysis of Methicillin-Resistant Staphylococcus aureus Nosocomial Infections

Antonio Olmos, Juan J. Camarena,* José M. Nogueira, Juan C. Navarro, Julia Risen, and Roberto Sánchez

Department of Microbiology, Hospital "Dr. Peset," E-46017 Valencia, Spain

Received 11 August 1997/Returned for modification 29 September 1997/Accepted 9 January 1998

The optimization of an arbitrarily primed PCR method for typing 96 methicillin-resistant Staphylococcus aureus (MRSA) isolates was compared with pulsed-field gel electrophoresis. Identical results in the differentiation of MRSA clones and identification of the main cluster that included 82 strains (88% of patients) were obtained by both techniques.

* Corresponding author. Mailing address: Department of Microbiology, Hospital "Dr. Peset," Gaspar Aguilar 90, E-46017 Valencia, Spain. Phone: 34-6-3987515. Fax: 34-6-3862576. E-mail: juan.camarena{at}uv.es.

Journal of Clinical Microbiology, April 1998, p. 1128-1134, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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