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Journal of Clinical Microbiology, April 1998, p. 1135-1136, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Bile-Esculin Test for Presumptive Identification of Enterococci and Streptococci: Effects of Bile Concentration, Inoculation Technique, and Incubation Time

C. Chuard1,dagger and L. B. Reller1,2,3,*

Clinical Microbiology Laboratory, Duke University Medical Center,1 and Departments of Pathology2 and Medicine,3 Duke University School of Medicine, Durham, North Carolina

Received 14 October 1997/Returned for modification 17 December 1997/Accepted 7 January 1998

The bile-esculin test is used to differentiate enterococci and group D streptococci from non-group D viridans group streptococci. The effects on test performance of the concentration of bile salts, inoculum, and duration of incubation were examined with 110 strains of enterococci, 30 strains of Streptococcus bovis, and 110 strains of non-group D viridans group streptococci. Optimal sensitivity (>99%) and specificity (97%) of the bile-esculin test can be obtained with a bile concentration of 40%, a standardized inoculum of 106 CFU, and incubation for 24 h.

* Corresponding author. Mailing address: Clinical Microbiology Laboratory, Box 3938, Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-6474. Fax: (919) 684-8519. E-mail: relle001{at}mc.duke.edu.

dagger Present address: Service de médecine, Hôpital Cantonal, 1708 Fribourg, Switzerland.

Journal of Clinical Microbiology, April 1998, p. 1135-1136, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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