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Journal of Clinical Microbiology, April 1998, p. 1151-1153, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Diagnosis of Mycoplasma pneumoniae Infection in Autopsy and Open-Lung Biopsy Tissues by Nested PCR

Deborah F. Talkington,1,* W. Lanier Thacker,1 David W. Keller,1,dagger and Jørgen S. Jensen2

Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 and Mycoplasma Laboratory, Statens Serum Institut, Copenhagen, Denmark2

Received 20 June 1997/Returned for modification 27 August 1997/Accepted 30 December 1997

A nested PCR specific for the Mycoplasma pneumoniae P1 gene was used to diagnose mycoplasma infection in two cohort patients with severe pneumonia within 24 h of tissue receipt. A postmortem diagnosis of M. pneumoniae infection was obtained for the first patient, who died without the collection of appropriate paired samples for serodiagnosis. An open-lung biopsy obtained from the second patient allowed a quick, definitive diagnosis and proper selection of therapy.

* Corresponding author. Mailing address: Mailstop CO2, Centers for Disease Control and Prevention, Atlanta, Georgia 30333. Phone: (404) 639-3918. Fax: (404) 639-3123. E-mail: DFT1{at}cdc.gov.

dagger Present address: New Mexico Department of Health, Santa Fe, NM 87502.

Journal of Clinical Microbiology, April 1998, p. 1151-1153, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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