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Journal of Clinical Microbiology, April 1998, p. 883-886, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of RapID Yeast Plus System with API 20C System for Identification of Common, New, and Emerging Yeast Pathogens

A. Espinel-Ingroff,1,* L. Stockman,2 G. Roberts,2 D. Pincus,3 J. Pollack,4 and J. Marler5,dagger

Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia1; Mayo Clinic, Rochester, Minnesota2; bioMérieux Vitek, Inc., Hazelwood, Missouri3; New York University Medical Center, New York, New York4; and Innovative Diagnostic Systems, L.P., Norcross, Georgia5

Received 27 October 1997/Returned for modification 28 November 1997/Accepted 26 December 1997

The ability to identify yeast isolates by the new enzymatic RapID Yeast Plus System was compared to the ability to identify yeast isolates by the API 20C system. A total of 447 yeast isolates representing Blastoschizomyces capitatus, 17 Candida spp., 5 Cryptococcus spp., Geotrichum spp., 2 Hanseniaspora spp., Hansenula anomala, Hansenula wingei, 3 Rhodotorula spp., Saccharomyces cerevisiae, Sporobolomyces salmonicolor, Trichosporon beigelii, and 2 Prototheca spp. were evaluated. Also, five quality control strains (Candida spp. and Cryptococcus laurentii) with well-documented reactivities by the RapID Yeast Plus System were used. Each isolate was evaluated by both methods with a 48-h culture grown at 30°C on Sabouraud dextrose agar (Emmons modification) by following the recommendations of the manufacturers. The RapID Yeast Plus System enzymatic reactions were read after 4 h of incubation, and the API 20C carbohydrate assimilation identification profiles were obtained after 72 h of incubation. There was good (95.7%) agreement between the identifications obtained by the two methods with the eight common Candida spp. and with Cryptococcus neoformans. The agreement was lower when the emerging Candida spp. and other yeast-like pathogens were tested (79.1 and 75.2%, respectively). These preliminary data suggest the potential utility of the RapID Yeast Plus System for use in the clinical laboratory for the rapid identification of common yeast pathogens as well as certain new and emerging species.

* Corresponding author. Mailing address: Division of Infectious Diseases, Medical College of Virginia/Medical Mycology Research Laboratory, Box 980049, 1101 E. Marshall St., Richmond, VA 23298-0049. Phone: (804) 828-9711. Fax: (804) 828-3097. E-mail: AVINGROFF{at}GEMS.VCU.EDU.

dagger Present address: VRG International Inc., Orlando, Fla.

Journal of Clinical Microbiology, April 1998, p. 883-886, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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