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Journal of Clinical Microbiology, April 1998, p. 883-886, Vol. 36, No. 4
Medical College of Virginia, Virginia
Commonwealth University, Richmond, Virginia1;
Mayo Clinic, Rochester, Minnesota2;
bioMérieux Vitek, Inc., Hazelwood,
Missouri3;
New York University Medical
Center, New York, New York4; and
Innovative Diagnostic Systems, L.P., Norcross,
Georgia5
Received 27 October 1997/Returned for modification 28 November
1997/Accepted 26 December 1997
The ability to identify yeast isolates by the new enzymatic RapID
Yeast Plus System was compared to the ability to identify yeast
isolates by the API 20C system. A total of 447 yeast isolates representing Blastoschizomyces capitatus, 17 Candida spp., 5 Cryptococcus spp.,
Geotrichum spp., 2 Hanseniaspora spp.,
Hansenula anomala, Hansenula wingei, 3 Rhodotorula spp., Saccharomyces cerevisiae, Sporobolomyces salmonicolor, Trichosporon
beigelii, and 2 Prototheca spp. were evaluated. Also,
five quality control strains (Candida spp. and
Cryptococcus laurentii) with well-documented reactivities by the RapID Yeast Plus System were used. Each isolate was evaluated by
both methods with a 48-h culture grown at 30°C on Sabouraud dextrose
agar (Emmons modification) by following the recommendations of the
manufacturers. The RapID Yeast Plus System enzymatic reactions were
read after 4 h of incubation, and the API 20C carbohydrate assimilation identification profiles were obtained after 72 h of
incubation. There was good (95.7%) agreement between the
identifications obtained by the two methods with the eight common
Candida spp. and with Cryptococcus neoformans.
The agreement was lower when the emerging Candida spp. and
other yeast-like pathogens were tested (79.1 and 75.2%, respectively).
These preliminary data suggest the potential utility of the RapID Yeast
Plus System for use in the clinical laboratory for the rapid
identification of common yeast pathogens as well as certain new and
emerging species.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Comparison of RapID Yeast Plus System with API 20C
System for Identification of Common, New, and Emerging Yeast
Pathogens
*
Corresponding author. Mailing address: Division of
Infectious Diseases, Medical College of Virginia/Medical Mycology
Research Laboratory, Box 980049, 1101 E. Marshall St., Richmond, VA
23298-0049. Phone: (804) 828-9711. Fax: (804) 828-3097. E-mail:
AVINGROFF{at}GEMS.VCU.EDU.
Present address: VRG International Inc., Orlando, Fla.
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