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Journal of Clinical Microbiology, April 1998, p. 975-978, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Mycobacterium tuberculosis Complex in Sputum Specimens by the Automated Roche Cobas Amplicor Mycobacterium Tuberculosis Test

Iiris Rajalahti,1,* Pauli Vuorinen,2 Markku M. Nieminen,1 and Ari Miettinen2

Departments of Pulmonary Diseases1 and Clinical Microbiology,2 Tampere University Hospital, FIN-33521, Tampere, Finland

Received 16 September 1997/Returned for modification 3 November 1997/Accepted 23 December 1997

Three hundred twenty-four sputum specimens from 151 patients with suspected active pulmonary tuberculosis were tested for the presence of the Mycobacterium tuberculosis complex with auramine fluorochrome stain and automated PCR assay (Roche Cobas Amplicor Mycobacterium Tuberculosis Test [MTB]). The results were compared with those of the conventional Löwenstein-Jensen tube culture and the BACTEC radiometer liquid culture. A total of 76 specimens from 32 patients were culture positive for M. tuberculosis. In addition, 37 specimens from 15 patients were smear and culture positive for other Mycobacterium species but negative by the present nucleic acid amplification method and thus were not included in the comparison. Compared with culture, the sensitivities, specificities, and positive and negative predictive values for acid-fast smear were 67, 98, 93, and 91% and those for the Cobas Amplicor MTB were 83, 99, 97, and 95%, respectively. When three consecutive sputum specimens per patient could be obtained, the sensitivity of the Cobas Amplicor MTB improved to 91%, whereas the sensitivity of the acid-fast smear remained unchanged.

* Corresponding author. Mailing address: Department of Pulmonary Diseases, Tampere University Hospital, Box 2000, FIN-33521, Tampere, Finland. Phone: 358-3-2473296. Fax: 358-3-2473006. E-mail: irajalahti{at}tays.fi.

Journal of Clinical Microbiology, April 1998, p. 975-978, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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