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Journal of Clinical Microbiology, April 1998, p. 983-985, Vol. 36, No. 4
Department of Clinical Microbiology,
Received 2 July 1997/Returned for modification 15 October
1997/Accepted 17 December 1997
Lactococcus garvieae (junior synonym Enterococcus
seriolicida) is an emerging zoonotic agent isolated from
economically important fish (rainbow trout and yellowtail), from
cattle, and from humans. Clindamycin susceptibility is the only
phenotypic test which can differentiate L. garvieae from
Lactococcus lactis, another emerging agent in humans. A PCR
assay for the identification of L. garvieae was developed
and resulted in an amplified fragment of 1,100 bp in size. The PCR
assay was shown to be specific to L. garvieae. The PCR
assay was positive for all the L. garvieae strains tested, which originated from three different continents (Asia, Australia, and
Europe). The PCR assay was negative for the phenotypically similar
L. lactis and for all the other fish pathogens tested, including Streptococcus iniae and Aeromonas
salmonicida. The PCR assay was applied to plasma obtained from
diseased animals and was found sensitive enough to detect bacteria from
1 µl of plasma. The PCR assay that was developed is the only
practical test besides the clindamycin test which can specifically
identify the zoonotic agent L. garvieae and which can
differentiate it from L. lactis.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Identification of Lactococcus garvieae
by PCR
Hadassah Medical School,
*
Corresponding author. Mailing address: Department of
Clinical Microbiology, The Hebrew University
Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel. Phone: 972-2-6758256. Fax:
972-2-6784010. E-mail: HB{at}cc.huji.ac.il.
Journal of Clinical Microbiology, April 1998, p. 983-985, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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