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Journal of Clinical Microbiology, April 1998, p. 983-985, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Lactococcus garvieae by PCR

Amir Zlotkin,1 Avi Eldar,2 Claudio Ghittino,3 and Herve Bercovier1,*

Department of Clinical Microbiology, The Hebrew University---Hadassah Medical School, Jerusalem 91120,1 and Department of Poultry and Fish Diseases, The Kimron Veterinary Institute, Beit Dagan 50250,2 Israel, and Experimental Institute for Zooprophylaxis, 10154 Torino, Italy3

Received 2 July 1997/Returned for modification 15 October 1997/Accepted 17 December 1997

Lactococcus garvieae (junior synonym Enterococcus seriolicida) is an emerging zoonotic agent isolated from economically important fish (rainbow trout and yellowtail), from cattle, and from humans. Clindamycin susceptibility is the only phenotypic test which can differentiate L. garvieae from Lactococcus lactis, another emerging agent in humans. A PCR assay for the identification of L. garvieae was developed and resulted in an amplified fragment of 1,100 bp in size. The PCR assay was shown to be specific to L. garvieae. The PCR assay was positive for all the L. garvieae strains tested, which originated from three different continents (Asia, Australia, and Europe). The PCR assay was negative for the phenotypically similar L. lactis and for all the other fish pathogens tested, including Streptococcus iniae and Aeromonas salmonicida. The PCR assay was applied to plasma obtained from diseased animals and was found sensitive enough to detect bacteria from 1 µl of plasma. The PCR assay that was developed is the only practical test besides the clindamycin test which can specifically identify the zoonotic agent L. garvieae and which can differentiate it from L. lactis.

* Corresponding author. Mailing address: Department of Clinical Microbiology, The Hebrew University---Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel. Phone: 972-2-6758256. Fax: 972-2-6784010. E-mail: HB{at}cc.huji.ac.il.

Journal of Clinical Microbiology, April 1998, p. 983-985, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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