This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by del Aguila, C.
Right arrow Articles by Visvesvara, G. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by del Aguila, C.
Right arrow Articles by Visvesvara, G. S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 1998, p. 1201-1208, Vol. 36, No. 5
0095-1137/98/$00.00+0

Ultrastructure, Immunofluorescence, Western Blot, and PCR Analysis of Eight Isolates of Encephalitozoon (Septata) intestinalis Established in Culture from Sputum and Urine Samples and Duodenal Aspirates of Five Patients with AIDS

C. del Aguila,1,2 G. P. Croppo,1 H. Moura,1,3 A. J. Da Silva,1 G. J. Leitch,4 D. M. Moss,1 S. Wallace,1 S. B. Slemenda,1 N. J. Pieniazek,1 and G. S. Visvesvara1,*

Division of Parasitic Diseases, Centers for Disease Control and Prevention,1 and Department of Physiology, Morehouse School of Medicine,4 Atlanta, Georgia; Unidad de Parasitología, Universidad San Pablo CEU, Madrid, Spain2; and Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro (UERJ), and Hospital Evandro Chagas, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil3

Received 30 October 1997/Returned for modification 8 December 1997/Accepted 26 January 1998

Microsporidia are ancient, intracellular, eukaryotic protozoan parasites that form spores and that lack mitochondria. Currently, as many as eight species included under six genera are known to infect humans, mostly patients with AIDS. Among these, Enterocytozoon bieneusi, the agent of gastrointestinal (GI) disease, is the most frequently identified microsporidian in clinical laboratories in the United States. Encephalitozoon (Septata) intestinalis, the agent that causes a disseminated infection including infection of the GI tract, is the second most frequently identified microsporidian parasite. In spite of this, not many isolates of E. intestinalis have been established in culture. We describe here the continuous cultivation of eight isolates of E. intestinalis obtained from different samples including the urine, sputum, and duodenal aspirate or biopsy specimens from five AIDS patients originating from California, Colorado, and Georgia. The specific identification was made on the bases of ultrastructural, antigenic, and PCR analyses.

* Corresponding author. Mailing address: Division of Parasitic Diseases, M.S.-F/13, Centers for Disease Control and Prevention, 4770 Buford Highway NE, Atlanta, GA 30341-3724. Phone: (770) 488-4417. Fax: (770) 488-4253. E-mail: GSV1{at}CDC.GOV.

Journal of Clinical Microbiology, May 1998, p. 1201-1208, Vol. 36, No. 5
0095-1137/98/$00.00+0


This article has been cited by other articles:

  • Rueda, C., Fenoy, S., Simon, F., del Aguila, C. (2008). Bobel-24 Activity against Cryptosporidium parvum in Cell Culture and in a SCID Mouse Model. Antimicrob. Agents Chemother. 52: 1150-1152 [Abstract] [Full Text]  
  • Mathis, A., Weber, R., Deplazes, P. (2005). Zoonotic Potential of the Microsporidia. Clin. Microbiol. Rev. 18: 423-445 [Abstract] [Full Text]  
  • Visvesvara, G. S. (2002). In Vitro Cultivation of Microsporidia of Clinical Importance. Clin. Microbiol. Rev. 15: 401-413 [Abstract] [Full Text]  
  • Garcia, L. S. (2002). Laboratory Identification of the Microsporidia. J. Clin. Microbiol. 40: 1892-1901 [Full Text]  
  • del Aguila, C., Moura, H., Fenoy, S., Navajas, R., Lopez-Velez, R., Li, L., Xiao, L., Leitch, G. J., da Silva, A., Pieniazek, N. J., Lal, A. A., Visvesvara, G. S. (2001). In Vitro Culture, Ultrastructure, Antigenic, and Molecular Characterization of Encephalitozoon cuniculi Isolated from Urine and Sputum Samples from a Spanish Patient with AIDS. J. Clin. Microbiol. 39: 1105-1108 [Abstract] [Full Text]  
  • Liguory, O., Fournier, S., Sarfati, C., Derouin, F., Molina, J.-M. (2000). Genetic Homology among Thirteen Encephalitozoon intestinalis Isolates Obtained from Human Immunodeficiency Virus-Infected Patients with Intestinal Microsporidiosis. J. Clin. Microbiol. 38: 2389-2391 [Abstract] [Full Text]  
  • Moss, D. M., Croppo, G. P., Wallace, S., Visvesvara, G. S. (1999). Flow Cytometric Analysis of Microsporidia Belonging to the Genus Encephalitozoon. J. Clin. Microbiol. 37: 371-375 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»