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Journal of Clinical Microbiology, May 1998, p. 1236-1239, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Ureaplasma urealyticum in Endotracheal Tube Aspirates from Neonates by PCR

S. Nelson,1,2,* A. Matlow,1,2 G. Johnson,1 C. Th'ng,3 M. Dunn,4,5 and P. Quinn1,2,4

Department of Pediatric Laboratory Medicine, The Hospital for Sick Children,1 Laboratory Services Branch, Ontario Ministry of Health,3 Department of Newborn and Developmental Pediatrics, Women's College Hospital,4 and Departments of Laboratory Medicine and Pathobiology2 and Pediatrics,5 The University of Toronto, Toronto, Canada

Received 19 June 1997/Returned for modification 20 August 1997/Accepted 16 February 1998

A PCR-based test was optimized for the detection of Ureaplasma urealyticum from neonatal respiratory specimens, with primers directed against the multiple-banded antigen gene (L. J. Teng, X. Zheng, J. I. Glass, H. Watson, J. Tsai, and G. H. Cassell, J. Clin. Microbiol. 32:1464-1469, 1994). Endotracheal tube aspirates (225) from 103 low-birth-weight neonates (<1,250 g) were taken, when possible, at days 0, 4, and 14 after birth and examined by culture and by PCR. Of 77 specimens positive by either method, 73 were detected by PCR and 60 were detected by culture. Overall, 36% of the neonates were positive for U. urealyticum by either method. Of 16 patients with PCR-positive-culture-negative results, 13 had positive cultures at another sampling point, and one additional patient had a twin with positive cultures. Of 11 patients with day 0 specimens positive by PCR alone, 9 subsequently became culture positive, demonstrating the utility of this test in early detection. Multiple serovars were present in over 50% of positive specimens, with serovars 3 and 14 in combination being most prevalent. The amplicon size generated from the specimen by PCR correctly predicted the biovars isolated in over 85% of positive specimens. Thus, this PCR test was valuable in allowing early detection of U. urealyticum in neonatal respiratory specimens, as well as in providing biovar information.

* Corresponding author. Present address: Pasteur Merieux Connaught Canada, 1755 Steeles Ave. W., Bldg. 81, North York, Ontario, Canada M2R 3T4. Phone: (416) 667-2700, ext. 3267. Fax: (416) 667-3004. E-mail: snelson{at}ca.pmc-vacc.com.

Journal of Clinical Microbiology, May 1998, p. 1236-1239, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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