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Journal of Clinical Microbiology, May 1998, p. 1330-1332, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparative Evaluation of National Committee for Clinical Laboratory Standards Broth Macrodilution and Agar Dilution Screening Methods for Testing Fluconazole Susceptibility of Cryptococcus neoformans

William R. Kirkpatrick,1 Robert K. McAtee,1 Sanjay G. Revankar,2 Annette W. Fothergill,2 Dora I. McCarthy,2 Michael G. Rinaldi,1,2 and Thomas F. Patterson1,3,*

Departments of Medicine1 and Pathology,2 University of Texas Health Science Center at San Antonio, and Audie Murphy Division, South Texas Veterans Health Care System,3 San Antonio, Texas 78284

Received 29 September 1997/Returned for modification 8 December 1997/Accepted 6 February 1998

A simple screening method for fluconazole susceptibility of Cryptococcus neoformans using 2% dextrose Sabouraud dextrose agar (SabDex) with fluconazole was compared to the National Committee for Clinical Laboratory Standards (NCCLS) broth macrodilution method. By this method, fluconazole-susceptible C. neoformans isolates are significantly smaller on medium with fluconazole than on fluconazole-free medium. Isolates with decreased susceptibility have normal-size colonies on medium containing fluconazole. The 48-h NCCLS broth macrodilution MICs (NCCLS MICs) for isolates with normal-size colonies on 8- or 16-µg/ml fluconazole plates were predicted to be >= 8 or >= 16 µg/ml, respectively. On medium with 16 µg of fluconazole per ml, all strains (84 of 84) for which the NCCLS MICs were <16 µg/ml were correctly predicted, as were all isolates (7 of 7) for which the MICs were >= 16 µg/ml. Agar dilution appears to be an effective screening method for fluconazole resistance in C. neoformans.

* Corresponding author. Mailing address: Department of Medicine, Division of Infectious Diseases, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78284-7881. Phone: (210) 567-4823. Fax: (210) 567-4670. E-mail: PATTERSON{at}UTHSCSA.EDU.

Journal of Clinical Microbiology, May 1998, p. 1330-1332, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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